Anti-ICAM3 antibody [ICAM3.1] (ab10804)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [ICAM3.1] (ab10804)

    Key features and details

    • Mouse monoclonal [ICAM3.1] to ICAM3
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    Overview

    • Product name

      Anti-ICAM3 antibody [ICAM3.1]
      See all ICAM3 primary antibodies

    • Description

      Mouse monoclonal [ICAM3.1] to ICAM3

    • Host species

      Mouse

    • Tested applications

      Suitable for: IHC-Pmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      ICAM 3Fc chimeric fusion protein.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab10804 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Notes
  • Application notes
    ELISA: Use at an assay dependent dilution.
    Flow Cyt: Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 1 µg/ml.
    IP: Use at an assay dependent dilution.
    RIA: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 66 kDa.
    ICC/IF: Use at an assay dependent dilution From (PubMed:17353362)
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.

    • Sequence similarities

      Belongs to the immunoglobulin superfamily. ICAM family.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

    • Post-translational
      modifications

      Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
      N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD50 antibody
        • CDW 50 antibody
        • CDw50 antibody
        • ICAM 3 antibody
        • ICAM-3 antibody
        • ICAM-R antibody
        • ICAM3 antibody
        • ICAM3_HUMAN antibody
        • ICAMR antibody
        • Intercellular adhesion molecule 3 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [ICAM3.1] (ab10804)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAM3 antibody [ICAM3.1] (ab10804)
        Ab10804 staining Human malignant stomach. Staining is localised to the membrane.
        Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
        Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      References (3)

      Publishing research using ab10804? Please let us know so that we can cite the reference in this datasheet.

      ab10804 has been referenced in 3 publications.

      • Mintz PJ  et al. Exploiting human CD34+ stem cell-conditioned medium for tissue repair. Mol Ther 22:149-59 (2014). PubMed: 23985698
      • Heasman SJ  et al. Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration. J Cell Biol 190:553-63 (2010). ICC/IF ; Human . PubMed: 20733052
      • Gérard A  et al. The Par polarity complex regulates Rap1- and chemokine-induced T cell polarization. J Cell Biol 176:863-75 (2007). PubMed: 17353362

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