Anti-ICAM3 antibody [ICAM3.1] (ab10804)

Ab10804 staining Human malignant stomach. Staining is localised to the membrane.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

ab10804 staining ICAM3 in Y27632-treated CEM T cells by Immunocytochemistry/ Immunofluorescence.
CEM T cells were fixed for 15 minutes with 4% paraformaldehyde. Cells were permeabilized with 0.1% Triton X-100, blocked with 1% BSA, and incubated with ab10804 (1/50), for 1 hour followed by Alexa Fluor 488–conjugated anti–mouse IgG secondary antibodies (1/200).