Overview

  • Product name

  • Description

    Rabbit polyclonal to ICAM4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 43-210 of Human ICAM4 (BC000046).

  • Positive control

    • Human fetal colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab112554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins.
  • Tissue specificity

    Erythrocytes.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. ICAM family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD242 antibody
    • CD242 antigen antibody
    • ICAM-4 antibody
    • Icam4 antibody
    • ICAM4_HUMAN antibody
    • Intercellular adhesion molecule 4 antibody
    • Landsteiner Wiener blood group glycoprotein antibody
    • Landsteiner-Wiener blood group glycoprotein antibody
    • LW antibody
    • LW blood group protein antibody
    see all

Images

  • ab112554, at a 1/100 dilution, staining ICAM4 in formalin-fixed, paraffin-embedded Human fetal colon tissue by Immunohistochemistry. Note: membrane staining.
  • Flow Cytometry analysis of ICAM4 in human endothelial precursor cells using ab112554 undiluted. The secondary used was an undiluted Allophycocyanin conjugated goat anti-rabbit polyclonal.
    Red line= ab112554
    Black line= isotype control

    See Abreview

References

This product has been referenced in:

  • Jackson AM  et al. Endothelial cell antibodies associated with novel targets and increased rejection. J Am Soc Nephrol 26:1161-71 (2015). Read more (PubMed: 25381426) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting Abcam.

Aswe carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More

Answer

Thank you for contacting Abcam.

Both ab67943 and ab112554 are intact IgG molecules containing the Fc region. I have looked through our catalogue and I cannot find anything that matches your requirements, but you could try searching http://www.biocompare.com.

Also we do not do custom synthesis orders, however the following companies can offer customer formulation

United States

Pocono Rabbit Farm and Laboratory (PRF&L)
PRF&L
PO Box 240
Canadensis, PA 18325
Tel: 800-622-6381 - Toll Free in the United States & Canada
Tel: 570-595-7108 - Worldwide
Fax: 570-595-9277
Website: www.prfal.com

Maine Biotechnology Services, Inc.
1037R Forest Ave.
Portland Maine 04103
Tel: 800-925-9476 or 207-797-5454
Fax: 207-797-5595
Website: www.mainebiotechnology.com

Cocalico Biologicals, Inc.
PO Box 265
449 Stevens Road
Reamstown, PA 17567-0265
Tel: 717-336-1990
Fax: 717-336-1993
Website: www.cocalicobiologicals.com

United Kingdom

Charles River Laboratories International, Inc.
Tel: +44 (0) 1843 823575
Website: www.criver.com

I hope this information is helpful to you. Please do not hesitate to contact us if you have any questions.

Read More
Application
Flow Cytometry
Fixation
none
Sample
Human Cell (endothelial precursor cells)
Specification
endothelial precursor cells
Gating Strategy
CD133 and CD202B
Permeabilization
No

Dr. A Jackson

Verified customer

Submitted Dec 05 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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