Key features and details
- Rabbit polyclonal to ICAM5
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Product nameAnti-ICAM5 antibody
See all ICAM5 primary antibodies
DescriptionRabbit polyclonal to ICAM5
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human, Pig
Recombinant fragment corresponding to Human ICAM5 aa 409-674. Expressed in E. coli. N-terminal GST-tag and His-tag.
RLDDSDCPRSWTWPEGPEQTLRCEARGNPEPSVHCARSDGGAVLALGLLG PVTRALSGTYRCKAANDQGEAVKDVTLTVEYAPALDSVGCPERITWLEGT EASLSCVAHGVPPPDVICVRSGELGAVIEGLLRVAREHAGTYRCEATNPR GSAAKNVAVTVEYGPRFEEPSCPSNWTWVEGSGRLFSCEVDGKPQPSVKC VGSGGATEGVLLPLAPPDPSPRAPRIPRVLAPGIYVCNATNRHGSVAKTV VVSAESPPEMDESTCP
Database link: Q9UMF0
- IHC-P: Human brain tissue. WB: Recombinant human ICAM5 protein. Mouse and pig cerebrum lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
RelevanceICAM5 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases.
Cellular localizationMembrane; single-pass type I membrane protein
- ICAM5 precursor antibody
- Intercellular adhesion molecule 5 antibody
- Intercellular adhesion molecule 5 precursor antibody
Formalin-fixed, paraffin-embedded human brain tissue stained for ICAM5 with ab232785 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-ICAM5 antibody (ab232785) at 1 µg/ml + Mouse cerebrum lysate
Anti-ICAM5 antibody (ab232785) at 1 µg/ml + Pig cerebrum lysate
Anti-ICAM5 antibody (ab232785) at 2 µg/ml + Recombinant human ICAM5 protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232785 has been referenced in 1 publication.
- Pei YP et al. ICAM5 as a Novel Target for Treating Cognitive Impairment in Fragile X Syndrome. J Neurosci 40:1355-1365 (2020). PubMed: 31882402