Key features and details
- Rabbit polyclonal to ICB1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ICB1 antibody
DescriptionRabbit polyclonal to ICB1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human ICB1 aa 62-177.
Database link: Q5TEJ8
- WB: A549 whole cell lysate. IHC-P: Human pancreatic cancer tissue. ICC/IF: NIH/3T3 cells.
This product was previously labelled as C1orf38
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 72 kDa.|
|IHC-P||1/200 - 1/500.|
|ICC/IF||1/50 - 1/200.|
FunctionMay constitute a control point in macrophage inflammatory response, promoting LPS-induced TNF production.
Tissue specificityExpressed in different endometrial adenocarcinoma cell lines and various other cell lines apart from the prostate cell line LNCaP and the ovarian cancer cell line BG1.
Sequence similaritiesBelongs to the themis family.
modificationsPhosphorylation at Tyr-632 is induced by LPS.
- Information by UniProt
- Basement membrane induced antibody
- Chromosome 1 open reading frame 38 antibody
- ICB 1 antibody
Anti-ICB1 antibody (ab236975) at 1/500 dilution + A549 (Human lung carcinoma cell line) whole cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 72 kDa
Paraffin-embedded human pancreatic cancer tissue stained for ICB1 using ab236975 at 1/100 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
NIH/3T3 (Mouse embryo fibroblast cell line) cells stained for ICB1 (green) using ab236975 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236975 has not yet been referenced specifically in any publications.