Overview

  • Product name

    Anti-ICB1 antibody
  • Description

    Rabbit polyclonal to ICB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human ICB1 aa 62-177.
    Database link: Q5TEJ8

  • Positive control

    • WB: A549 whole cell lysate. IHC-P: Human pancreatic cancer tissue. ICC/IF: NIH/3T3 cells.
  • General notes

     This product was previously labelled as C1orf38

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab236975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 72 kDa.
IHC-P 1/200 - 1/500.
ICC/IF 1/50 - 1/200.

Target

  • Function

    May constitute a control point in macrophage inflammatory response, promoting LPS-induced TNF production.
  • Tissue specificity

    Expressed in different endometrial adenocarcinoma cell lines and various other cell lines apart from the prostate cell line LNCaP and the ovarian cancer cell line BG1.
  • Sequence similarities

    Belongs to the themis family.
  • Post-translational
    modifications

    Phosphorylation at Tyr-632 is induced by LPS.
  • Information by UniProt
  • Database links

  • Alternative names

    • Basement membrane induced antibody
    • Chromosome 1 open reading frame 38 antibody
    • ICB 1 antibody
    • ICB1 antibody
    • Induced by contact to basement membrane 1 protein antibody
    • Protein ICB 1 antibody
    • Protein ICB-1 antibody
    • Protein THEMIS2 antibody
    • THEMIS2 antibody
    • THMS2_HUMAN antibody
    • Thymocyte expressed molecule involved in selection protein 2 antibody
    • Thymocyte-expressed molecule involved in selection protein 2 antibody
    see all

Images

  • Anti-ICB1 antibody (ab236975) at 1/500 dilution + A549 (Human lung carcinoma cell line) whole cell lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 72 kDa

  • Paraffin-embedded human pancreatic cancer tissue stained for ICB1 using ab236975 at 1/100 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • NIH/3T3 (Mouse embryo fibroblast cell line) cells stained for ICB1 (green) using ab236975 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

References

ab236975 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236975.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up