Overview

  • Product name

  • Description

    Rabbit polyclonal to ICER
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Recombinant fragment corresponding to Human ICER aa 93-241.

  • Positive control

    • NT2D1, A549, HeLa and Jurkat whole cell lysate (ab7899); HeLa cells.
  • General notes

    Protein previously labeled as CREM.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 37 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation.
  • Tissue specificity

    Expressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes.
  • Sequence similarities

    Belongs to the bZIP family.
    Contains 1 bZIP (basic-leucine zipper) domain.
    Contains 1 KID (kinase-inducible) domain.
  • Post-translational
    modifications

    Isoform 9 is ubiquitinated by CDC34 and RAD6B in order to be degraded by the proteasome.
    Stimulated by phosphorylation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • cAMP response element modulator antibody
    • cAMP responsive element modulator antibody
    • cAMP-responsive element modulator antibody
    • CREM antibody
    • CREM-2 antibody
    • CREM_HUMAN antibody
    • hCREM 2 antibody
    • hCREM-2 antibody
    • hCREM2 antibody
    • ICER antibody
    • Inducible cAMP early repressor antibody
    • Inducible cAMP early repressor ICER antibody
    • MGC111110 antibody
    • MGC17881 antibody
    • MGC41893 antibody
    see all

Images

  • Anti-ICER antibody (ab153723) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg

    Predicted band size: 37 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells, labeling ICER with ab153723 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

References

ab153723 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Western blot
Sample
Rat Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
30 µg
Specification
heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Mrs. Andrea Geelhaar

Verified customer

Submitted Feb 08 2019

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