Western blot abreview for Anti-ICER antibody

Average
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
30 µg
Specification
heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST buffer
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: POD-F(ab)2-anti-rabbit-IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: HRP polymer

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 34 kDa Non-specific: 40, 45, 60 kDa
Positive control
loading control: GAPDH

Additional data

Additional Notes
The left ventricle of the heart was lysed and used.

Mrs. Andrea Geelhaar

Verified customer

Submitted Feb 08 2019

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