Anti-ICER antibody (ab230543)
Key features and details
- Rabbit polyclonal to ICER
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-ICER antibody
See all ICER primary antibodies -
Description
Rabbit polyclonal to ICER -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig -
Immunogen
Synthetic peptide corresponding to Human ICER (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: Q03060 -
Positive control
- WB: HeLa and RAW 264.7 whole cell lysate. IHC-P: Human testis and brain tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100.
|
|
WB |
1/500 - 1/1000. Predicted molecular weight: 39 kDa.
|
Notes |
---|
IHC-P
1/100. |
WB
1/500 - 1/1000. Predicted molecular weight: 39 kDa. |
Target
-
Function
Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation. -
Tissue specificity
Expressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes. -
Sequence similarities
Belongs to the bZIP family.
Contains 1 bZIP (basic-leucine zipper) domain.
Contains 1 KID (kinase-inducible) domain. -
Post-translational
modificationsIsoform 9 is ubiquitinated by CDC34 and RAD6B in order to be degraded by the proteasome.
Stimulated by phosphorylation. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 540605 Cow
- Entrez Gene: 403887 Dog
- Entrez Gene: 1390 Human
- Entrez Gene: 12916 Mouse
- Entrez Gene: 448969 Pig
- Entrez Gene: 25620 Rat
- Omim: 123812 Human
- SwissProt: Q1LZH5 Cow
see all -
Alternative names
- cAMP response element modulator antibody
- cAMP responsive element modulator antibody
- cAMP-responsive element modulator antibody
see all
Images
-
Formalin-fixed, paraffin-embedded human testis tissue stained for ICER using ab230543 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human brain (cerebellum) tissue stained for ICER using ab230543 at 1/100 dilution in immunohistochemical analysis.
-
All lanes :
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Predicted band size: 39 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab230543 has been referenced in 1 publication.
- Vermeulen M et al. Generation of Organized Porcine Testicular Organoids in Solubilized Hydrogels from Decellularized Extracellular Matrix. Int J Mol Sci 20:N/A (2019). PubMed: 31684200