Key features and details
- Rabbit polyclonal to ICER
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ICER antibody
See all ICER primary antibodies
DescriptionRabbit polyclonal to ICER
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig
Synthetic peptide corresponding to Human ICER (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: Q03060
- WB: HeLa and RAW 264.7 whole cell lysate. IHC-P: Human testis and brain tissue.
Protein previously labeled as CREM.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 39 kDa.|
FunctionTranscriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation.
Tissue specificityExpressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes.
Sequence similaritiesBelongs to the bZIP family.
Contains 1 bZIP (basic-leucine zipper) domain.
Contains 1 KID (kinase-inducible) domain.
modificationsIsoform 9 is ubiquitinated by CDC34 and RAD6B in order to be degraded by the proteasome.
Stimulated by phosphorylation.
- Information by UniProt
- cAMP response element modulator antibody
- cAMP responsive element modulator antibody
- cAMP-responsive element modulator antibody
Formalin-fixed, paraffin-embedded human testis tissue stained for ICER using ab230543 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human brain (cerebellum) tissue stained for ICER using ab230543 at 1/100 dilution in immunohistochemical analysis.
All lanes :
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Predicted band size: 39 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230543 has been referenced in 1 publication.
- Vermeulen M et al. Generation of Organized Porcine Testicular Organoids in Solubilized Hydrogels from Decellularized Extracellular Matrix. Int J Mol Sci 20:N/A (2019). PubMed: 31684200