Anti-ICOS antibody [7E.17G9] (ab95630)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ICOS antibody [7E.17G9]
See all ICOS primary antibodies -
Description
Rat monoclonal [7E.17G9] to ICOS -
Host species
Rat -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Mouse ICOS cDNA and ICOS hexahistidine fusion protein.
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Positive control
- Three day ConA activated BALB/c splenocytes or Mouse splenocyte suspensions
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
7E.17G9 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.06-1µg for 105-8 cells. A 100 µl volume is recommended for staining the cell sample.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Target
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Function
Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. -
Tissue specificity
Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus. -
Involvement in disease
Immunodeficiency, common variable, 1 -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 54167 Mouse
- SwissProt: Q9WVS0 Mouse
- Unigene: 42044 Mouse
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Alternative names
- Activation inducible lymphocyte immunomediatory molecule antibody
- Activation-inducible lymphocyte immunomediatory molecule antibody
- AILIM antibody
see all
Images
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![Flow Cytometry - Anti-ICOS antibody [7E.17G9] (ab95630)](https://www.abcam.com/ps/products/95/ab95630/Images/ab95630-109900-anti-icos-antibody-7e17g9-flow-cytometry.jpg)
Flow Cytometry - Anti-ICOS antibody [7E.17G9] (ab95630)Staining of unstimulated (left) and 3 day ConA activated (right) BALB/c splenocytes with 0.06 µg of Rat IgG2b Isotype Control (open histogram) or 0.06 µg of ab95630 (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.
Protocols
Datasheets and documents
References
ab95630 has not yet been referenced specifically in any publications.
