For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    icos-antibody-7e17g9-ab95630.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Adaptive Immunity T Cells CD
Share by email

Anti-ICOS antibody [7E.17G9] (ab95630)

  • Datasheet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Flow Cytometry - Anti-ICOS antibody [7E.17G9] (ab95630)

    Key features and details

    • Rat monoclonal [7E.17G9] to ICOS
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Isotype: IgG2b

    You may also be interested in

    Protein
    Product image
    Recombinant Mouse ICOS protein (His tag) (ab215625)
    Secondary
    Product image
    Goat Anti-Rat IgG H&L (HRP) (ab97057)

    View more associated products

    Overview

    • Product name

      Anti-ICOS antibody [7E.17G9]
      See all ICOS primary antibodies
    • Description

      Rat monoclonal [7E.17G9] to ICOS
    • Host species

      Rat
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Mouse ICOS cDNA and ICOS hexahistidine fusion protein.

    • Positive control

      • Three day ConA activated BALB/c splenocytes or Mouse splenocyte suspensions
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.87% Sodium chloride, PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      7E.17G9
    • Isotype

      IgG2b
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Isotype control

      • Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control (ab18541)
    • Recombinant Protein

      • Recombinant Mouse ICOS protein (His tag) (ab215625)

    Applications

    Our Abpromise guarantee covers the use of ab95630 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 0.06-1µg for 105-8 cells.

    A 100 µl volume is recommended for staining the cell sample.

     

     

     

    ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes.
    • Tissue specificity

      Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.
    • Involvement in disease

      Immunodeficiency, common variable, 1
    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications

      N-glycosylated.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession Q9Y6W8 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 54167 Mouse
      • SwissProt: Q9WVS0 Mouse
      • Unigene: 42044 Mouse
      • Alternative names

        • Activation inducible lymphocyte immunomediatory molecule antibody
        • Activation-inducible lymphocyte immunomediatory molecule antibody
        • AILIM antibody
        • CD278 antibody
        • CD278 antigen antibody
        • CD28-related Protein-1 antibody
        • CRP1 antibody
        • CVID1 antibody
        • ICOS antibody
        • ICOS_HUMAN antibody
        • Inducible costimulator antibody
        • Inducible T cell co stimulator antibody
        • Inducible T-cell costimulator antibody
        • MGC39850 antibody
        see all

      Images

      • Flow Cytometry - Anti-ICOS antibody [7E.17G9] (ab95630)
        Flow Cytometry - Anti-ICOS antibody [7E.17G9] (ab95630)
        Staining of unstimulated (left) and 3 day ConA activated (right) BALB/c splenocytes with 0.06 µg of Rat IgG2b Isotype Control (open histogram) or 0.06 µg of ab95630 (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab95630? Please let us know so that we can cite the reference in this datasheet.

      ab95630 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab95630.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.