Recombinant Anti-ICOS antibody [SP98] (ab105227)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)](https://www.abcam.com/ps/products/105/ab105227/Images/ab105227-5-AntiICOS-antibody-SP98-IHC-human-tonsil.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP98] to ICOS
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ICOS antibody [SP98]
See all ICOS primary antibodies -
Description
Rabbit monoclonal [SP98] to ICOS -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human ICOS aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9Y6W8 -
Positive control
- IHC-P: Human lymph node, pancreatic adenocarcinoma, thymus and tonsil tissue. IHC-Fr: Frozen human tonsil tissue sections
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP98 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
1/100. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at RT for 20 min.
|
| IHC-Fr | (1) |
Use a concentration of 5 µg/ml.
|
| Notes |
|---|
|
IHC-P
1/100. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at RT for 20 min. |
|
IHC-Fr
Use a concentration of 5 µg/ml. |
Target
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Function
Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. -
Tissue specificity
Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus. -
Involvement in disease
Immunodeficiency, common variable, 1 -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 29851 Human
- Entrez Gene: 54167 Mouse
- Omim: 604558 Human
- SwissProt: Q9Y6W8 Human
- SwissProt: Q9WVS0 Mouse
- Unigene: 56247 Human
- Unigene: 42044 Mouse
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Alternative names
- Activation inducible lymphocyte immunomediatory molecule antibody
- Activation-inducible lymphocyte immunomediatory molecule antibody
- AILIM antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling ICOS with ab105227 at 1/100 dilution (0.28 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 30 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Sporadically positive staining in the germinal center of human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab105227 for 30 mins at room temperature. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)](https://www.abcam.com/ps/products/105/ab105227/Images/ab105227-294310-anti-icos-antibody-sp98-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)Formalin-fixed, paraffin-embedded human tonsil tissue stained for ICOS using ab105227 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)](https://www.abcam.com/ps/products/105/ab105227/Images/ab105227-117741-anti-icos-antibody-sp98-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)ab105227, at 1/100 dilution, staining ICOS in formalin-fixed, paraffin-embedded Human reactive lymph node tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)](https://www.abcam.com/ps/products/105/ab105227/Images/ab105227-294312-anti-icos-antibody-sp98-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for ICOS using ab105227 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)](https://www.abcam.com/ps/products/105/ab105227/Images/ab105227-294311-anti-icos-antibody-sp98-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)Formalin-fixed, paraffin-embedded human thymus tissue stained for ICOS using ab105227 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Frozen sections) - Anti-ICOS antibody [SP98] (ab105227)](https://www.abcam.com/ps/products/105/ab105227/Images/ab105227-7-antiicossp98immunohistochemistryfrozentonsilhuman.jpg)
Immunohistochemistry (Frozen sections) - Anti-ICOS antibody [SP98] (ab105227)IHC image of ICOS staining in a section of frozen normal human tonsil* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab105227, 5ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
![Immunohistochemistry (Frozen sections) - Anti-ICOS antibody [SP98] (ab105227)](https://www.abcam.com/ps/products/105/ab105227/Images/ab105227-8-antiicossp98immunohistochemistryfrozenplacentahuman.jpg)
Immunohistochemistry (Frozen sections) - Anti-ICOS antibody [SP98] (ab105227)Negative control image: IHC image of ICOS staining in a section of frozen normal human placenta* performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab105227, 5ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre -
![Anti-ICOS antibody [SP98] (ab105227)](https://www.abcam.com/ps/products/105/ab105227/Images/ab105227-6-benefits-of-recombinant-antibodies.png)
Anti-ICOS antibody [SP98] (ab105227)
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab105227 has been referenced in 10 publications.
- Pontarini E et al. Unique expansion of IL-21+ Tfh and Tph cells under control of ICOS identifies Sjögren's syndrome with ectopic germinal centres and MALT lymphoma. Ann Rheum Dis 79:1588-1599 (2020). PubMed: 32963045
- Gerlach MM et al. Bone Marrow Infiltration of Angioimmunoblastic T-Cell Lymphoma: Identification and Prognostic Impact of Histologic Patterns and Diagnostic Application of Ancillary Phenotypic and Molecular Analyses. Arch Pathol Lab Med 144:602-611 (2020). PubMed: 31556696
- Huang M et al. The Influence of Immune Heterogeneity on the Effectiveness of Immune Checkpoint Inhibitors in Multifocal Hepatocellular Carcinomas. Clin Cancer Res 26:4947-4957 (2020). PubMed: 32527942
- de Pádua Covas Lage LA et al. Proliferative, pro-inflammatory, and angiogenesis regulator gene expression profile defines prognosis in different histopathological subtypes of nodal peripheral T-cell lymphoma. Oncotarget 10:5136-5151 (2019). PubMed: 31497245
- Le KS et al. CXCR5 and ICOS expression identifies a CD8 T-cell subset with TFH features in Hodgkin lymphomas. Blood Adv 2:1889-1900 (2018). PubMed: 30087107
- Hurabielle C et al. Eruption of lymphocyte recovery with atypical lymphocytes mimicking a primary cutaneous T-cell lymphoma: a series of 12 patients. Hum Pathol 71:100-108 (2018). IHC-P ; Human . PubMed: 29107664
- Yang H et al. Frequency and distribution of CD4+CXCR5+ follicular B helper T cells within involved tissues in IgG4-related ophthalmic disease. Mol Med Rep 16:9512-9520 (2017). PubMed: 29039547
- Le KS et al. Follicular B Lymphomas Generate Regulatory T Cells via the ICOS/ICOSL Pathway and Are Susceptible to Treatment by Anti-ICOS/ICOSL Therapy. Cancer Res 76:4648-60 (2016). PubMed: 27246829
- Song Y et al. Increased frequency of thymic T follicular helper cells in myasthenia gravis patients with thymoma. J Thorac Dis 8:314-22 (2016). IHC-P ; Human . PubMed: 27076925
- Zhang Y et al. The clinical impact of ICOS signal in colorectal cancer patients. Oncoimmunology 5:e1141857 (2016). IHC . PubMed: 27467961