Recombinant Anti-ICOS antibody [SP98] (ab105227)

Rabbit recombinant monoclonal ICOS antibody [SP98]. Validated in IHC and tested in Human. Cited in 5 publication(s). Independently reviewed in 1 review(s).


  • Product name
    Anti-ICOS antibody [SP98]
    See all ICOS primary antibodies
  • Description
    Rabbit monoclonal [SP98] to ICOS
  • Host species
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human ICOS aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9Y6W8

  • Positive control
    • IHC-P: Human lymph node, pancreatic adenocarcinoma, thymus and tonsil tissue.
  • General notes



    This product is a recombinant rabbit monoclonal antibody.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Protein A/G purified
  • Purification notes
    Purified from TCS by protein A/G.
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab105227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at RT for 20 min.


  • Function
    Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes.
  • Tissue specificity
    Activated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.
  • Involvement in disease
    Immunodeficiency, common variable, 1
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activation inducible lymphocyte immunomediatory molecule antibody
    • Activation-inducible lymphocyte immunomediatory molecule antibody
    • AILIM antibody
    • CD278 antibody
    • CD278 antigen antibody
    • CD28-related Protein-1 antibody
    • CRP1 antibody
    • CVID1 antibody
    • ICOS antibody
    • ICOS_HUMAN antibody
    • Inducible costimulator antibody
    • Inducible T cell co stimulator antibody
    • Inducible T-cell costimulator antibody
    • MGC39850 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling ICOS with ab105227 at 1/100 dilution (0.28 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 30 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Sporadically positive staining in the germinal center of human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab105227 for 30 mins at room temperature.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for ICOS using ab105227 at 1/100 dilution in immunohistochemical analysis.

  • ab105227, at 1/100 dilution, staining ICOS in formalin-fixed, paraffin-embedded Human reactive lymph node tissue by Immunohistochemistry.
  • Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for ICOS using ab105227 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human thymus tissue stained for ICOS using ab105227 at 1/100 dilution in immunohistochemical analysis.


This product has been referenced in:
  • Hurabielle C  et al. Eruption of lymphocyte recovery with atypical lymphocytes mimicking a primary cutaneous T-cell lymphoma: a series of 12 patients. Hum Pathol 71:100-108 (2018). IHC-P ; Human . Read more (PubMed: 29107664) »
  • Yang H  et al. Frequency and distribution of CD4+CXCR5+ follicular B helper T cells within involved tissues in IgG4-related ophthalmic disease. Mol Med Rep 16:9512-9520 (2017). Read more (PubMed: 29039547) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Frozen sections)
Mouse Tissue sections (Spleen)
Blocking step
Casein as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 29 2012


Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab105227 in IHC-Fr and mouse. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-Fr and mouseand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site:

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