Anti-ICOS antibody [SP98] (ab105227)
- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-ICOS antibody [SP98]
See all ICOS primary antibodies -
DescriptionRabbit monoclonal [SP98] to ICOS
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human ICOS aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9Y6W8 -
Positive control
- Human Tonsil and Reactive Lymph Node
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General notes
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from TCS by protein A/G.
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ClonalityMonoclonal
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Clone numberSP98
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab105227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform antigen retrieval before commencing with IHC staining protocol by boiling tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at RT for 20 min. |
Target
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FunctionEnhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes.
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Tissue specificityActivated T-cells. Highly expressed on tonsillar T-cells, which are closely associated with B-cells in the apical light zone of germinal centers, the site of terminal B-cell maturation. Expressed at lower levels in thymus, lung, lymph node and peripheral blood leukocytes. Expressed in the medulla of fetal and newborn thymus.
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Involvement in diseaseImmunodeficiency, common variable, 1
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Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
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Post-translational
modificationsN-glycosylated. -
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 29851 Human
- Entrez Gene: 54167 Mouse
- Omim: 604558 Human
- SwissProt: Q9Y6W8 Human
- SwissProt: Q9WVS0 Mouse
- Unigene: 56247 Human
- Unigene: 42044 Mouse
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Alternative names
- Activation inducible lymphocyte immunomediatory molecule antibody
- Activation-inducible lymphocyte immunomediatory molecule antibody
- AILIM antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)](https://www.abcam.com/ps/products/105/ab105227/Images/ICOS-Primary-antibodies-ab105227-1.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)ab105227, at 1/100 dilution, staining ICOS in formalin-fixed, paraffin-embedded Human reactive lymph node tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)](https://www.abcam.com/ps/products/105/ab105227/Images/ab105227-294312-ab1052273.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for ICOS using ab105227 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)](https://www.abcam.com/ps/products/105/ab105227/Images/ab105227-294311-ab1052272.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)Formalin-fixed, paraffin-embedded human thymus tissue stained for ICOS using ab105227 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)](https://www.abcam.com/ps/products/105/ab105227/Images/ab105227-294310-ab1052271.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (ab105227)Formalin-fixed, paraffin-embedded human tonsil tissue stained for ICOS using ab105227 at 1/100 dilution in immunohistochemical analysis.
Protocols
References
This product has been referenced in:
- Hurabielle C et al. Eruption of lymphocyte recovery with atypical lymphocytes mimicking a primary cutaneous T-cell lymphoma: a series of 12 patients. Hum Pathol 71:100-108 (2018). IHC-P ; Human . Read more (PubMed: 29107664) »
- Le KS et al. Follicular B Lymphomas Generate Regulatory T Cells via the ICOS/ICOSL Pathway and Are Susceptible to Treatment by Anti-ICOS/ICOSL Therapy. Cancer Res 76:4648-60 (2016). Read more (PubMed: 27246829) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"