Overview

  • Product name

    Anti-ICOS Ligand/ICOSL antibody
    See all ICOS Ligand/ICOSL primary antibodies
  • Description

    Rabbit polyclonal to ICOS Ligand/ICOSL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse ICOS Ligand/ICOSL aa 300 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q9JHJ8

  • Positive control

    • This antibody gave a positive signal in Mouse Heart and Kidney tissue lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal spleen.
  • General notes

     This product was previously labelled as ICOS Ligand

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab138354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 33 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
  • Tissue specificity

    Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • B7 H2 antibody
    • B7 homolog 2 antibody
    • B7 homologue 2 antibody
    • B7 like protein Gl50 antibody
    • B7 related protein 1 antibody
    • B7-H2 antibody
    • B7-like protein Gl50 antibody
    • B7-related protein 1 antibody
    • B7H2 antibody
    • B7RP 1 antibody
    • B7RP-1 antibody
    • B7RP1 antibody
    • CD 275 antibody
    • CD275 antibody
    • CD275 antigen antibody
    • GL 50 antibody
    • GL50 antibody
    • ICOS L antibody
    • ICOS LG antibody
    • ICOS ligand antibody
    • ICOSL antibody
    • ICOSL_HUMAN antibody
    • Icoslg antibody
    • Inducible T cell co stimulator ligand antibody
    • KIAA0653 antibody
    • LICOS antibody
    • Transmembrane protein B7 H2 ICOS ligand antibody
    see all

Images

  • IHC image of ICOS Ligand/ICOSL staining in Mouse normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab138354, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-ICOS Ligand/ICOSL antibody (ab138354) at 1 µg/ml

    Lane 1 : Heart (Mouse) Tissue Lysate
    Lane 2 : Kidney (Mouse) Tissue Lysate
    Lane 3 : Heart (Mouse) Tissue Lysate with Immunising peptide at 1 µg/ml
    Lane 4 : Kidney (Mouse) Tissue Lysate with Immunising peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 28 kDa (possible non-specific binding)


    Exposure time: 12 minutes


    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab138354 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:

  • Knox T  et al. Selective HDAC6 inhibitors improve anti-PD-1 immune checkpoint blockade therapy by decreasing the anti-inflammatory phenotype of macrophages and down-regulation of immunosuppressive proteins in tumor cells. Sci Rep 9:6136 (2019). Read more (PubMed: 30992475) »
  • Roth M  et al. Pelargonium sidoides radix extract EPs 7630 reduces rhinovirus infection through modulation of viral binding proteins on human bronchial epithelial cells. PLoS One 14:e0210702 (2019). Read more (PubMed: 30707726) »
See all 4 Publications for this product

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