Key features and details
- Rabbit polyclonal to ICOS Ligand/ICOSL
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ICOS Ligand/ICOSL antibody
See all ICOS Ligand/ICOSL primary antibodies
DescriptionRabbit polyclonal to ICOS Ligand/ICOSL
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment (His-T7-tag) corresponding to Human ICOS Ligand/ICOSL aa 23-249. (Expressed in E.coli).
KEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSS LENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGF QEVLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWI NKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLL QQNLTVGSQTGNDIGERDKITENPVST
Database link: O75144
- WB: Human serum; Human liver and lung lysate; Jurkat whole cell lysate (ab7899); Recombinant human ICOS Ligand/ICOSL protein. IHC-P: Human tonsil tissue.
This product was previously labelled as ICOS Ligand
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 33 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionLigand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
Tissue specificityIsoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells.
Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- B7 H2 antibody
- B7 homolog 2 antibody
- B7 homologue 2 antibody
Formalin-fixed, paraffin-embedded human tonsil tissue stained for ICOS Ligand/ICOSL using ab233151 at 20 µg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-ICOS Ligand/ICOSL antibody (ab233151) at 2 µg/ml
Lane 1 : Human serum
Lane 2 : Human liver tissue lysate
Lane 3 : Human lung tissue lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
All lanes : HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDa
Anti-ICOS Ligand/ICOSL antibody (ab233151) at 2 µg/ml + Recombinant human ICOS Ligand/ICOSL protein
Predicted band size: 33 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233151 has not yet been referenced specifically in any publications.