Recombinant Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ICOS Ligand/ICOSL antibody [EPR20240]
See all ICOS Ligand/ICOSL primary antibodies -
Description
Rabbit monoclonal [EPR20240] to ICOS Ligand/ICOSL -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human ICOS Ligand/ICOSL aa 1-300. The exact sequence is proprietary.
Database link: O75144 -
Positive control
- WB: His-tagged recombinant human ICOS ligand/ICOSL protein; Untreated and PNGase F treated JAR whole cell lysates; Untreated and tumor necrosis factor-alpha (TNF-alpha, ab9642) 200 unit/ml for 24 hours treated HUVEC whole cell lysates. IP: JAR whole cell lysate.
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General notes
This product was previously labelled as ICOS Ligand
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20240 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab209262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 33 kDa). | |
| IP | 1/40. |
Target
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Function
Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. -
Tissue specificity
Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 23308 Human
- Omim: 605717 Human
- SwissProt: O75144 Human
- Unigene: 14155 Human
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Alternative names
- B7 H2 antibody
- B7 homolog 2 antibody
- B7 homologue 2 antibody
see all
Images
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![Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262)](https://www.abcam.com/ps/products/209/ab209262/Images/ab209262-274047-anti-icos-ligand-antibody-epr20240-western-blot.jpg)
Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262)Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262) at 1/20000 dilution + His-tagged recombinant human ICOS ligand/ICOSL protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 33 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262)](https://www.abcam.com/ps/products/209/ab209262/Images/ab209262-274046-anti-icos-ligand-antibody-epr20240-western-blot.jpg)
Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262)All lanes : Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262) at 1/1000 dilution
Lane 1 : Untreated JAR (Human placenta choriocarcinoma cell line) whole cell lysate
Lane 2 : JAR whole cell lysate treated with PNGase F
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 33 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
ICOS ligand/ICOSL is highly glycosylated (PMID 11983910).
Observed band sizes: 60 kDa (glycosylated form); 35 kDa (non-glycosylated form).
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![Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262)](https://www.abcam.com/ps/products/209/ab209262/Images/ab209262-274045-anti-icos-ligand-antibody-epr20240-western-blot.jpg)
Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262)All lanes : Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262) at 1/1000 dilution
Lane 1 : Untreated HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lane 2 : HUVEC whole cell lysate treated with tumor necrosis factor-a (TNF-alpha, ab9642) 200 unit/ml for 24 hours
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The level of ICOS Ligand/ICOSL expression can be elevated by TNF-alpha treatment (PMID 11983910).
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![Immunoprecipitation - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262)](https://www.abcam.com/ps/products/209/ab209262/Images/ab209262-274044-anti-icos-ligand-antibody-epr20240-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262)ICOS Ligand/ICOSL was immunoprecipitated from 0.35 mg of JAR (Human placenta choriocarcinoma cell line) whole cell lysate with ab209262 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab209262 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: JAR whole cell lysate, 10 μg (Input).
Lane 2: ab209262 IP in JAR whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209262 in JAR whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Datasheets and documents
References
ab209262 has not yet been referenced specifically in any publications.
