Recombinant
RabMAb

Recombinant Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262)

Overview

  • Product name

    Anti-ICOS Ligand/ICOSL antibody [EPR20240]
    See all ICOS Ligand/ICOSL primary antibodies
  • Description

    Rabbit monoclonal [EPR20240] to ICOS Ligand/ICOSL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ICOS Ligand/ICOSL aa 1-300. The exact sequence is proprietary.
    Database link: O75144

  • Positive control

    • WB: His-tagged recombinant human ICOS ligand/ICOSL protein; Untreated and PNGase F treated JAR whole cell lysates; Untreated and tumor necrosis factor-alpha (TNF-alpha, ab9642) 200 unit/ml for 24 hours treated HUVEC whole cell lysates. IP: JAR whole cell lysate.
  • General notes

     This product was previously labelled as ICOS Ligand

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20240
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab209262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 33 kDa).
IP 1/40.

Target

  • Function

    Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
  • Tissue specificity

    Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • B7 H2 antibody
    • B7 homolog 2 antibody
    • B7 homologue 2 antibody
    • B7 like protein Gl50 antibody
    • B7 related protein 1 antibody
    • B7-H2 antibody
    • B7-like protein Gl50 antibody
    • B7-related protein 1 antibody
    • B7H2 antibody
    • B7RP 1 antibody
    • B7RP-1 antibody
    • B7RP1 antibody
    • CD 275 antibody
    • CD275 antibody
    • CD275 antigen antibody
    • GL 50 antibody
    • GL50 antibody
    • ICOS L antibody
    • ICOS LG antibody
    • ICOS ligand antibody
    • ICOSL antibody
    • ICOSL_HUMAN antibody
    • Icoslg antibody
    • Inducible T cell co stimulator ligand antibody
    • KIAA0653 antibody
    • LICOS antibody
    • Transmembrane protein B7 H2 ICOS ligand antibody
    see all

Images

  • Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262) at 1/20000 dilution + His-tagged recombinant human ICOS ligand/ICOSL protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • All lanes : Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262) at 1/1000 dilution

    Lane 1 : Untreated JAR (Human placenta choriocarcinoma cell line) whole cell lysate
    Lane 2 : JAR whole cell lysate treated with PNGase F

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    ICOS ligand/ICOSL is highly glycosylated (PMID 11983910).

    Observed band sizes: 60 kDa (glycosylated form); 35 kDa (non-glycosylated form).

  • All lanes : Anti-ICOS Ligand/ICOSL antibody [EPR20240] (ab209262) at 1/1000 dilution

    Lane 1 : Untreated HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
    Lane 2 : HUVEC whole cell lysate treated with tumor necrosis factor-a (TNF-alpha, ab9642) 200 unit/ml for 24 hours

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The level of ICOS Ligand/ICOSL expression can be elevated by TNF-alpha treatment (PMID 11983910).

  • ICOS Ligand/ICOSL was immunoprecipitated from 0.35 mg of JAR (Human placenta choriocarcinoma cell line) whole cell lysate with ab209262 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab209262 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: JAR whole cell lysate, 10 μg (Input).

    Lane 2: ab209262 IP in JAR whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209262 in JAR whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

References

ab209262 has not yet been referenced specifically in any publications.

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