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    icos-ligandicosl-antibody-epr6071-ab124972.pdf

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Immunology Adaptive Immunity T Cells Non-CD
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RecombinantRabMAb

Recombinant Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)

  • Datasheet
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Abpromise

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Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)
  • OI-RD Scanning - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)
  • Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6071] to ICOS Ligand/ICOSL
  • Suitable for: WB
  • Reacts with: Human

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Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Recombinant human ICOS Ligand/ICOSL protein (Fc Chimera Active) (ab214966)

View more associated products

Overview

  • Product name

    Anti-ICOS Ligand/ICOSL antibody [EPR6071]
    See all ICOS Ligand/ICOSL primary antibodies
  • Description

    Rabbit monoclonal [EPR6071] to ICOS Ligand/ICOSL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ICOS Ligand/ICOSL aa 250-350 (intracellular). The exact sequence is proprietary.

  • Positive control

    • HL60, HuT78 and HUVEC (untreated or treated with TNF-alpha) lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as ICOS Ligand

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 8.80 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6071
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD

Associated products

  • Alternative Versions

    • Anti-ICOS Ligand/ICOSL antibody [EPR6071] - BSA and Azide free (ab248080)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human ICOS Ligand/ICOSL protein (Fc Chimera Active) (ab214966)

Applications

Our Abpromise guarantee covers the use of ab124972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 63 - 72 kDa (predicted molecular weight: 33 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
    • Tissue specificity

      Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily. BTN/MOG family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession O75144 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 23308 Human
      • Omim: 605717 Human
      • SwissProt: O75144 Human
      • Unigene: 14155 Human
      • Alternative names

        • B7 H2 antibody
        • B7 homolog 2 antibody
        • B7 homologue 2 antibody
        • B7 like protein Gl50 antibody
        • B7 related protein 1 antibody
        • B7-H2 antibody
        • B7-like protein Gl50 antibody
        • B7-related protein 1 antibody
        • B7H2 antibody
        • B7RP 1 antibody
        • B7RP-1 antibody
        • B7RP1 antibody
        • CD 275 antibody
        • CD275 antibody
        • CD275 antigen antibody
        • GL 50 antibody
        • GL50 antibody
        • ICOS L antibody
        • ICOS LG antibody
        • ICOS ligand antibody
        • ICOSL antibody
        • ICOSL_HUMAN antibody
        • Icoslg antibody
        • Inducible T cell co stimulator ligand antibody
        • KIAA0653 antibody
        • LICOS antibody
        • Transmembrane protein B7 H2 ICOS ligand antibody
        see all

      Images

      • Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)
        Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)
        All lanes : Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972) at 1/1000 dilution

        Lane 1 : HL60 cell lysate
        Lane 2 : HuT78 cell lysate
        Lane 3 : HUVEC cell lysate, treated with TNF-alpha
        Lane 4 : HUVEC cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

        Predicted band size: 33 kDa

      • OI-RD Scanning - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)
        OI-RD Scanning - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)
        Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab124972? Please let us know so that we can cite the reference in this datasheet.

      ab124972 has been referenced in 1 publication.

      • Tao YW  et al. Association between Increased Inducible Costimulator/Inducible Costimulator Ligand Expression with Bone Destruction in Apical Periodontitis. J Endod 45:890-897 (2019). PubMed: 31153660

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab124972.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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