Product nameAnti-ICOS Ligand/ICOSL antibody [EPR6071]
See all ICOS Ligand/ICOSL primary antibodies
DescriptionRabbit monoclonal [EPR6071] to ICOS Ligand/ICOSL
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human ICOS Ligand/ICOSL aa 250-350 (intracellular). The exact sequence is proprietary.
- HL60, HuT78 and HUVEC (untreated or treated with TNF-alpha) lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as ICOS Ligand
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 8.80 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab124972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 63 - 72 kDa (predicted molecular weight: 33 kDa).|
|ICC/IF||1/50 - 1/100.|
FunctionLigand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.
Tissue specificityIsoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells.
Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- B7 H2 antibody
- B7 homolog 2 antibody
- B7 homologue 2 antibody
All lanes : Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972) at 1/1000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : HuT78 cell lysate
Lane 3 : HUVEC cell lysate, treated with TNF-alpha
Lane 4 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 33 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124972 has not yet been referenced specifically in any publications.