Recombinant Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)
![Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)](https://www.abcam.com/ps/products/124/ab124972/Images/ab124972-139061-anti-icos-ligand-antibody-epr6071-western-blot.jpg "Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6071] to ICOS Ligand/ICOSL
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-ICOS Ligand/ICOSL antibody [EPR6071]
See all ICOS Ligand/ICOSL primary antibodies -
Description
Rabbit monoclonal [EPR6071] to ICOS Ligand/ICOSL -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human ICOS Ligand/ICOSL aa 250-350 (intracellular). The exact sequence is proprietary.
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Positive control
- HL60, HuT78 and HUVEC (untreated or treated with TNF-alpha) lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as ICOS Ligand
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 8.80 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6071 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 63 - 72 kDa (predicted molecular weight: 33 kDa). |
Target
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Function
Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. -
Tissue specificity
Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 23308 Human
- Omim: 605717 Human
- SwissProt: O75144 Human
- Unigene: 14155 Human
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Alternative names
- B7 H2 antibody
- B7 homolog 2 antibody
- B7 homologue 2 antibody
see all
Images
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Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)All lanes : Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972) at 1/1000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : HuT78 cell lysate
Lane 3 : HUVEC cell lysate, treated with TNF-alpha
Lane 4 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 33 kDa -
![OI-RD Scanning - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)](https://www.abcam.com/ps/products/124/ab124972/Images/ab124972-139060-anti-icos-ligand-antibody-epr6071.jpg)
OI-RD Scanning - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972) -
![Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)](https://www.abcam.com/ps/products/124/ab124972/Images/ab124972-3-benefits-of-recombinant-antibodies.png)
Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)
Datasheets and documents
References (1)
ab124972 has been referenced in 1 publication.
- Tao YW et al. Association between Increased Inducible Costimulator/Inducible Costimulator Ligand Expression with Bone Destruction in Apical Periodontitis. J Endod 45:890-897 (2019). PubMed: 31153660
