Recombinant
RabMAb

Recombinant Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)

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Overview

  • Product name

    Anti-ICOS Ligand/ICOSL antibody [EPR6071]
    See all ICOS Ligand/ICOSL primary antibodies

  • Description

    Rabbit monoclonal [EPR6071] to ICOS Ligand/ICOSL

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human ICOS Ligand/ICOSL aa 250-350 (intracellular). The exact sequence is proprietary.

  • Positive control

    • HL60, HuT78 and HUVEC (untreated or treated with TNF-alpha) lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as ICOS Ligand

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 8.80 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6071

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 63 - 72 kDa (predicted molecular weight: 33 kDa).
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    • Function

      Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.

    • Tissue specificity

      Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells.

    • Sequence similarities

      Belongs to the immunoglobulin superfamily. BTN/MOG family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • B7 H2 antibody
        • B7 homolog 2 antibody
        • B7 homologue 2 antibody
        • B7 like protein Gl50 antibody
        • B7 related protein 1 antibody
        • B7-H2 antibody
        • B7-like protein Gl50 antibody
        • B7-related protein 1 antibody
        • B7H2 antibody
        • B7RP 1 antibody
        • B7RP-1 antibody
        • B7RP1 antibody
        • CD 275 antibody
        • CD275 antibody
        • CD275 antigen antibody
        • GL 50 antibody
        • GL50 antibody
        • ICOS L antibody
        • ICOS LG antibody
        • ICOS ligand antibody
        • ICOSL antibody
        • ICOSL_HUMAN antibody
        • Icoslg antibody
        • Inducible T cell co stimulator ligand antibody
        • KIAA0653 antibody
        • LICOS antibody
        • Transmembrane protein B7 H2 ICOS ligand antibody
        see all

      Images

      • Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)
        Western blot - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)
        All lanes : Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972) at 1/1000 dilution

        Lane 1 : HL60 cell lysate
        Lane 2 : HuT78 cell lysate
        Lane 3 : HUVEC cell lysate, treated with TNF-alpha
        Lane 4 : HUVEC cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

        Predicted band size: 33 kDa

      • Other - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)
        Other - Anti-ICOS Ligand/ICOSL antibody [EPR6071] (ab124972)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      ab124972 has not yet been referenced specifically in any publications.

      Publishing research using ab124972? Please let us know so that we can cite the reference in this datasheet.

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