Recombinant Anti-ICOS Ligand/ICOSL antibody [HK53] (ab245678)
![Immunocytochemistry/ Immunofluorescence - Anti-ICOS Ligand/ICOSL antibody [HK53] (ab245678)](https://www.abcam.com/ps/products/245/ab245678/Images/ab245678-336920-anti-icos-ligand-antibody-hk53-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ICOS Ligand/ICOSL antibody [HK53] (ab245678)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HK53] to ICOS Ligand/ICOSL
- Suitable for: ICC/IF
- Reacts with: Mouse
Overview
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Product name
Anti-ICOS Ligand/ICOSL antibody [HK53]
See all ICOS Ligand/ICOSL primary antibodies -
Description
Rabbit monoclonal [HK53] to ICOS Ligand/ICOSL -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Mouse
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Immunogen
Tissue, cells or virus corresponding to ICOS Ligand/ICOSL. Mouse B7h-transfected L cells. (fused with immune splenocytes with P3U1 myeloma cells and screened for binding to mouse B7h-transfected NRK cells).
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Positive control
- ICC/IF: Mouse splenocytes.
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General notes
This product was previously labelled as ICOS Ligand
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HK53 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab245678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
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| Notes |
|---|
|
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. -
Tissue specificity
Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 50723 Mouse
- SwissProt: Q9JHJ8 Mouse
- Unigene: 17819 Mouse
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Alternative names
- B7 H2 antibody
- B7 homolog 2 antibody
- B7 homologue 2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ICOS Ligand/ICOSL antibody [HK53] (ab245678)
PFA fixed, 0.15% Triton permeabilized mouse splenocytes labeling ICOS Ligand/ICOSL using ab245678 at 10 µg/ml for 1 hour (green) followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue).
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![Anti-ICOS Ligand/ICOSL antibody [HK53] (ab245678)](https://www.abcam.com/ps/products/245/ab245678/Images/ab245678-3-benefits-of-recombinant-antibodies.png)
Anti-ICOS Ligand/ICOSL antibody [HK53] (ab245678)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab245678 has not yet been referenced specifically in any publications.