Recombinant Anti-ICOS Ligand/ICOSL antibody [HK53] (ab245678)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ICOS Ligand/ICOSL antibody [HK53]
See all ICOS Ligand/ICOSL primary antibodies -
Description
Rabbit monoclonal [HK53] to ICOS Ligand/ICOSL -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to ICOS Ligand/ICOSL. Mouse B7h-transfected L cells. (fused with immune splenocytes with P3U1 myeloma cells and screened for binding to mouse B7h-transfected NRK cells).
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Positive control
- ICC/IF: Mouse splenocytes.
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General notes
This product was previously labelled as ICOS Ligand
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HK53 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab245678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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Function
Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. -
Tissue specificity
Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 50723 Mouse
- SwissProt: Q9JHJ8 Mouse
- Unigene: 17819 Mouse
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Alternative names
- B7 H2 antibody
- B7 homolog 2 antibody
- B7 homologue 2 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-ICOS Ligand/ICOSL antibody [HK53] (ab245678)](https://www.abcam.com/ps/products/245/ab245678/Images/ab245678-336920-anti-icos-ligand-antibody-hk53-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ICOS Ligand/ICOSL antibody [HK53] (ab245678)PFA fixed, 0.15% Triton permeabilized mouse splenocytes labeling ICOS Ligand/ICOSL using ab245678 at 10 µg/ml for 1 hour (green) followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), in ICC/IF. The nuclear counter stain is DAPI (blue).
References
ab245678 has not yet been referenced specifically in any publications.
