Anti-Id1 antibody [2456C1a] (ab66495)
Key features and details
- Mouse monoclonal [2456C1a] to Id1
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Id1 antibody [2456C1a]
See all Id1 primary antibodies -
Description
Mouse monoclonal [2456C1a] to Id1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human Id1 (internal sequence).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (~95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
2456C1a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa. |
Target
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Function
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Developmental stage
Expression correlates with proliferation in some types of cells. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Alternative names
- bHLHb24 antibody
- Class B basic helix-loop-helix protein 24 antibody
- dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein) antibody
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Datasheets and documents
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Datasheet download
References (4)
ab66495 has been referenced in 4 publications.
- He Y et al. A noncanonical AR addiction drives enzalutamide resistance in prostate cancer. Nat Commun 12:1521 (2021). PubMed: 33750801
- Ohara RA et al. Citrullinated Inhibitor of DNA Binding 1 Is a Novel Autoantigen in Rheumatoid Arthritis. Arthritis Rheumatol 71:1241-1251 (2019). PubMed: 30861322
- Hu XM et al. ID1 contributes to cell growth invasion and migration in salivary adenoid cystic carcinoma. Mol Med Rep N/A:N/A (2017). PubMed: 29039489
- Snyder AD et al. Expression pattern of id proteins in medulloblastoma. Pathol Oncol Res 19:437-46 (2013). PubMed: 23397264