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Mouse polyclonal to Id1
Predicted to work with:
Mouse, Rat, Sheep, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
Full-length protein corresponding to amino acids 1-155 of Human Id1 (NP_002156.2).
Lysate from Id1 transfected 293T cells; HeLa cells
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Constituent: 100% PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa.
Use a concentration of 10 µg/ml. ab168256 must be purified for ICC/IF application.
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.
Contains 1 basic helix-loop-helix (bHLH) domain.
Expression correlates with proliferation in some types of cells.
Information by UniProt
Class B basic helix-loop-helix protein 24 antibody
dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein) antibody
Western blot - Anti-Id1 antibody (ab168256)
All lanes : Anti-Id1 antibody (ab168256) at 1/500 dilution Lane 1 : ID1-transfected 293T cell lysate Lane 2 : Non-transfected 293T cell lysate Lysates/proteins at 15 µl per lane. Predicted band size: 16 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Id1 antibody (ab168256)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling Id1 using ab168256 at 10 µg/ml. Cells should be permeabilized by incubating for 15 minutes on ice with 2 mL of 0.1% Triton X-100 in PBS prior to staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"