Overview

  • Product name
  • Description
    Mouse polyclonal to Id1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Full-length protein corresponding to amino acids 1-155 of Human Id1 (NP_002156.2).

  • Positive control
    • Lysate from Id1 transfected 293T cells; HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab168256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa.
ICC/IF Use a concentration of 10 µg/ml. ab168256 must be purified for ICC/IF application.

Target

  • Function
    ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Developmental stage
    Expression correlates with proliferation in some types of cells.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb24 antibody
    • Class B basic helix-loop-helix protein 24 antibody
    • dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein) antibody
    • DNA binding protein inhibitor ID 1 antibody
    • DNA binding protein inhibitor ID1 antibody
    • DNA-binding protein inhibitor ID-1 antibody
    • Dominant negative helix loop helix protein antibody
    • ID 1 antibody
    • ID antibody
    • ID1 antibody
    • ID1_HUMAN antibody
    • Inhibitor of Differentiation 1 antibody
    • Inhibitor of DNA binding 1 antibody
    • inhibitor of DNA binding 1, dominant negative helix-loop-helix protein antibody
    see all

Images

  • All lanes : Anti-Id1 antibody (ab168256) at 1/500 dilution

    Lane 1 : ID1-transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Predicted band size: 16 kDa

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling Id1 using ab168256 at 10 µg/ml. Cells should be permeabilized by incubating for 15 minutes on ice with 2 mL of 0.1% Triton X-100 in PBS prior to staining.

References

This product has been referenced in:
  • Li Y  et al. Effect and mechanism of RNAi targeting WWTR1 on biological activity of gastric cancer cells SGC7901. Mol Med Rep 17:2853-2860 (2018). Read more (PubMed: 29207147) »
  • Li L  et al. siRNA-mediated knockdown of ID1 disrupts Nanog- and Oct-4-mediated cancer stem cell-likeness and resistance to chemotherapy in gastric cancer cells. Oncol Lett 13:3014-3024 (2017). Read more (PubMed: 28529558) »
See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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