Anti-Id1 antibody (ab230679)
Key features and details
- Rabbit polyclonal to Id1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Id1 antibody
See all Id1 primary antibodies -
Description
Rabbit polyclonal to Id1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit -
Immunogen
Synthetic peptide within Human Id1 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P41134 -
Positive control
- WB: HeLa and HepG2 whole cell lysate (ab7900); Mouse and rat liver whole cell lysates. IHC-P: Human placenta tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 16 kDa.
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IHC-P |
1/100.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 16 kDa. |
IHC-P
1/100. |
Target
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Function
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Developmental stage
Expression correlates with proliferation in some types of cells. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3397 Human
- Entrez Gene: 15901 Mouse
- Entrez Gene: 25261 Rat
- Omim: 600349 Human
- SwissProt: P41134 Human
- SwissProt: P20067 Mouse
- SwissProt: P41135 Rat
- Unigene: 504609 Human
see all -
Alternative names
- bHLHb24 antibody
- Class B basic helix-loop-helix protein 24 antibody
- dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein) antibody
see all
Images
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All lanes : Anti-Id1 antibody (ab230679) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : Mouse liver whole cell lysate
Lane 4 : Rat liver whole cell lysate
Developed using the ECL technique.
Predicted band size: 16 kDa -
Formalin-fixed, paraffin-embedded human placenta tissue stained for Id1 with ab230679 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab230679 has been referenced in 5 publications.
- Chen H et al. BMP9-ID1 Signaling Activates HIF-1α and VEGFA Expression to Promote Tumor Angiogenesis in Hepatocellular Carcinoma. Int J Mol Sci 23:N/A (2022). PubMed: 35163396
- Tang Y et al. ID1 marks the tumorigenesis of pancreatic ductal adenocarcinoma in mouse and human. Sci Rep 12:13555 (2022). PubMed: 35941362
- Xie T et al. BMPER Ameliorates Renal Fibrosis by Inhibiting Tubular Dedifferentiation and Fibroblast Activation. Front Cell Dev Biol 9:608396 (2021). PubMed: 33644047
- El-Huneidi W et al. The Coffee Diterpene, Kahweol, Ameliorates Pancreatic β-Cell Function in Streptozotocin (STZ)-Treated Rat INS-1 Cells through NF-kB and p-AKT/Bcl-2 Pathways. Molecules 26:N/A (2021). PubMed: 34500601
- Meng L et al. Vital Roles of Gremlin-1 in Pulmonary Arterial Hypertension Induced by Systemic-to-Pulmonary Shunts. J Am Heart Assoc 9:e016586 (2020). PubMed: 32750294