Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS
Concentration information loading...
PurityProtein G purified
Purification notesab53545 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Our Abpromise guarantee covers the use of ab53545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Detects a band of approximately 32 kDa (predicted molecular weight: 15 kDa).|
|Dot blot||Use at an assay dependent dilution.|
FunctionID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
Tissue specificityHighly expressed in early fetal tissues, including those of the central nervous system.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Developmental stageFound in most early fetal tissues but not in the corresponding mature tissues.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- bHLHb26 antibody
- Cell growth inhibiting gene 8 antibody
- class B basic helix loop helix protein 26 antibody
Anti-ID2 antibody [2457C5a] (ab53545) + immunised recombinant protein
Predicted band size: 15 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
ab53545 has not yet been referenced specifically in any publications.