Anti-ID2 antibody [4E12G5] (ab166708)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [4E12G5] (ab166708)
  • Western blot - Anti-ID2 antibody [4E12G5] (ab166708)
  • Flow Cytometry - Anti-ID2 antibody [4E12G5] (ab166708)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [4E12G5] (ab166708)

Key features and details

  • Mouse monoclonal [4E12G5] to ID2
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-ID2 antibody [4E12G5]
    See all ID2 primary antibodies

  • Description

    Mouse monoclonal [4E12G5] to ID2

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Chimpanzee, Cynomolgus monkey, Orangutan

  • Immunogen

    Purified recombinant full length protein, corresponding to amino acids 1-134 of Human ID2, expressed in E. coli (UniProt: Q02363).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human recombinant ID2 protein; SK N SH cells; Human breast cancer and rectum cancer tissues.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab166708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 17.3 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.

  • Tissue specificity

    Highly expressed in early fetal tissues, including those of the central nervous system.

  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.

  • Developmental stage

    Found in most early fetal tissues but not in the corresponding mature tissues.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • bHLHb26 antibody
    • Cell growth inhibiting gene 8 antibody
    • class B basic helix loop helix protein 26 antibody
    • Class B basic helix-loop-helix protein 26 antibody
    • DNA binding protein inhibitor ID 2 antibody
    • DNA binding protein inhibitor ID2 antibody
    • DNA-binding protein inhibitor ID-2 antibody
    • GIG 8 antibody
    • GIG8 antibody
    • Helix loop helix protein ID2 antibody
    • ID2 antibody
    • ID2_HUMAN antibody
    • ID2A antibody
    • ID2H antibody
    • Inhibitor of differentiation 2 antibody
    • Inhibitor of DNA binding 2 antibody
    • Inhibitor of DNA binding 2, dominant negative helix loop helix protein antibody
    • MGC26389 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [4E12G5] (ab166708)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [4E12G5] (ab166708)
    Immunohistochemical analysis of paraffin embedded, DAB-stained Human breast cancer tissue labeling ID2 using ab166708 at a 1/200 dilution.
  • Western blot - Anti-ID2 antibody [4E12G5] (ab166708)
    Western blot - Anti-ID2 antibody [4E12G5] (ab166708)
    Anti-ID2 antibody [4E12G5] (ab166708) at 1/500 dilution + recombinant Human ID2 protein at 1 µg

    Predicted band size: 17.3 kDa

  • Flow Cytometry - Anti-ID2 antibody [4E12G5] (ab166708)
    Flow Cytometry - Anti-ID2 antibody [4E12G5] (ab166708)
    Flow cytometric analysis of ID2-labeled SK N SH cells (green) and negative control cells (red), using ab166708 at a 1/200 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [4E12G5] (ab166708)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ID2 antibody [4E12G5] (ab166708)
    Immunohistochemical analysis of paraffin embedded, DAB-stained Human rectum cancer tissue labeling ID2 using ab166708 at a 1/200 dilution.

References (7)

Publishing research using ab166708? Please let us know so that we can cite the reference in this datasheet.

ab166708 has been referenced in 7 publications.

  • Yuan M  et al. Phospholipase C (PLC)e Promotes Androgen Receptor Antagonist Resistance via the Bone Morphogenetic Protein (BMP)-6/SMAD Axis in a Castration-Resistant Prostate Cancer Cell Line. Med Sci Monit 25:4438-4449 (2019). PubMed: 31201297
  • Liu T  et al. miR-27a promotes endothelial-mesenchymal transition in hypoxia-induced pulmonary arterial hypertension by suppressing BMP signaling. Life Sci 227:64-73 (2019). PubMed: 31004656
  • Fan H  et al. Effect of Notch1 gene on remyelination in multiple sclerosis in mouse models of acute demyelination. J Cell Biochem N/A:N/A (2018). PubMed: 30010211
  • Hwang SM  et al. Inflammation-induced Id2 promotes plasticity in regulatory T cells. Nat Commun 9:4736 (2018). PubMed: 30413714
  • Le Dréau G  et al. E proteins sharpen neurogenesis by modulating proneural bHLH transcription factors' activity in an E-box-dependent manner. Elife 7:N/A (2018). PubMed: 30095408
  • Feng Y  et al. Alpha-1-antitrypsin functions as a protective factor in preeclampsia through activating Smad2 and inhibitor of DNA binding 4. Oncotarget 8:113002-113012 (2017). PubMed: 29348884
  • Wang S  et al. Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation. Cell 171:201-216.e18 (2017). PubMed: 28844693

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ID2 antibody [4E12G5]

 Below Average
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA (pH 9.0)
Permeabilization
No
Specification
Mammary gland
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
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Jinwoo Seong

Verified customer

Submitted Jul 11 2016

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