Overview

  • Product name
  • Description
    Rabbit polyclonal to ID2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human ID2 aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q02363

  • Positive control
    • Breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab52093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

1/50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Target

  • Function
    ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
  • Tissue specificity
    Highly expressed in early fetal tissues, including those of the central nervous system.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Developmental stage
    Found in most early fetal tissues but not in the corresponding mature tissues.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHb26 antibody
    • Cell growth inhibiting gene 8 antibody
    • class B basic helix loop helix protein 26 antibody
    • Class B basic helix-loop-helix protein 26 antibody
    • DNA binding protein inhibitor ID 2 antibody
    • DNA binding protein inhibitor ID2 antibody
    • DNA-binding protein inhibitor ID-2 antibody
    • GIG 8 antibody
    • GIG8 antibody
    • Helix loop helix protein ID2 antibody
    • ID2 antibody
    • ID2_HUMAN antibody
    • ID2A antibody
    • ID2H antibody
    • Inhibitor of differentiation 2 antibody
    • Inhibitor of DNA binding 2 antibody
    • Inhibitor of DNA binding 2, dominant negative helix loop helix protein antibody
    • MGC26389 antibody
    see all

Images

  • Staining of ID2 in human breast carcinoma by immunohistochemistry, using ab52093 (1/50). (Formalin fixed, Paraffin embedded section).

References

This product has been referenced in:
  • Nikolic MZ  et al. Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids. Elife 6:N/A (2017). IHC-Fr . Read more (PubMed: 28665271) »
  • Snyder AD  et al. Expression pattern of id proteins in medulloblastoma. Pathol Oncol Res 19:437-46 (2013). Read more (PubMed: 23397264) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human embryonic lung - 7 weeks post conception)
Permeabilization
Yes - 10 min 0.3% triton post-sectioning
Specification
Human embryonic lung - 7 weeks post conception
Blocking step
1% BSA, 5% normal donkey serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Ms. Emma Rawlins

Verified customer

Submitted May 24 2017

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Mouse Cell (bEnd.3)
Specification
bEnd.3
Permeabilization
Yes - 0.1% Triton X100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 14 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample
Human Cell (HUVEC)
Specification
HUVEC
Permeabilization
Yes - 0.1% Triton X100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 02 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (breast cancer, prostate cancer)
Specification
breast cancer, prostate cancer
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted Feb 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up