Anti-ID2 antibody (ab85990)
Key features and details
- Rabbit polyclonal to ID2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-ID2 antibody
See all ID2 primary antibodies -
Description
Rabbit polyclonal to ID2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment -
Immunogen
3 synthetic peptides (Human) conjugated to KLH
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
|
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| WB | (1) |
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 15 kDa).
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| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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WB
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 15 kDa). |
Target
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Function
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression. -
Tissue specificity
Highly expressed in early fetal tissues, including those of the central nervous system. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Developmental stage
Found in most early fetal tissues but not in the corresponding mature tissues. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3398 Human
- Entrez Gene: 15902 Mouse
- Omim: 600386 Human
- SwissProt: Q02363 Human
- SwissProt: P41136 Mouse
- Unigene: 180919 Human
- Unigene: 34871 Mouse
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Alternative names
- bHLHb26 antibody
- Cell growth inhibiting gene 8 antibody
- class B basic helix loop helix protein 26 antibody
see all
Images
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Western blot - Anti-ID2 antibody (ab85990)All lanes : Anti-ID2 antibody (ab85990) at 1/1000 dilution
Lane 1 : Uninduced (negative control) culture of E.coli
Lane 2 : Induced culture of E.coli
Secondary
All lanes : anti-Rabbit IgG HRP conjugated at 1/1000 dilution
Predicted band size: 15 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Predicted molecular weight of Recombinant ID2:29 kDa (15 kDa + another 14 kDa for the tag).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab85990 has been referenced in 4 publications.
- Johnson JR et al. Microarray and pathway analysis of two COMMA-Dß derived clones reveal important differences relevant to their developmental capacity in-vivo. Oncotarget 10:2118-2135 (2019). PubMed: 31040905
- Cheng X et al. KIAA1429 regulates the migration and invasion of hepatocellular carcinoma by altering m6A modification of ID2 mRNA. Onco Targets Ther 12:3421-3428 (2019). PubMed: 31118692
- Florio F et al. Sustained Expression of Negative Regulators of Myelination Protects Schwann Cells from Dysmyelination in a Charcot-Marie-Tooth 1B Mouse Model. J Neurosci 38:4275-4287 (2018). PubMed: 29610440
- Ladle BH et al. De novo DNA methylation by DNA methyltransferase 3a controls early effector CD8+ T-cell fate decisions following activation. Proc Natl Acad Sci U S A 113:10631-6 (2016). Flow Cyt ; Mouse . PubMed: 27582468