Key features and details
- Mouse monoclonal [OTI10C3] to ID2
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-ID2 antibody [OTI10C3]
See all ID2 primary antibodies
DescriptionMouse monoclonal [OTI10C3] to ID2
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Cynomolgus monkey, Orangutan
Recombinant full length protein corresponding to Human ID2 aa 1-134. Protein expressed in E.coli. NP_002157
MKAFSPVRSVRKNSLSDHSLGISRSKTPVDDPMSLLYNMNDCYSKLKELV PSIPQNKKVSKMEILQHVIDYILDLQIALDSHPTIVSLHHQRPGQNQASR TPLTTLNTDISILSLQASEFPSELMSNDSKALCG
Database link: Q02363
- IHC-P: Human liver, bladder carcinoma and liver carcinoma tissue. ICC/IF: HeLa cells. WB: pCMV6-ENTRY ID2 cDNA transfected HEK-293T cell lysate. Flow Cyt: SH-SY5Y cells.
The clone number has been updated from 10C3 to OTI10C3, both clone numbers name the same clone.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, PBS
Concentration information loading...
Purification notesPurified from cell culture supernatant by affinity chromatography
Our Abpromise guarantee covers the use of ab90055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 15 kDa.|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||Use at an assay dependent concentration.|
FunctionID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
Tissue specificityHighly expressed in early fetal tissues, including those of the central nervous system.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Developmental stageFound in most early fetal tissues but not in the corresponding mature tissues.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- bHLHb26 antibody
- Cell growth inhibiting gene 8 antibody
- class B basic helix loop helix protein 26 antibody
All lanes : Anti-ID2 antibody [OTI10C3] (ab90055) at 1/500 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells were transfected with the pCMV6-ENTRY control
Lane 2 : HEK-293T cells were transfected with the pCMV6-ENTRY ID2 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 15 kDa
Paraffin-embedded human bladder carcinoma tissue stained for ID2 with ab90055 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for ID2 using ab90055 at a 1/50 dilution in ICC/IF.
Paraffin-embedded human liver tissue stained for ID2 with ab90055 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Paraffin-embedded human liver carcinoma tissue stained for ID2 with ab90055 at a 1/50 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
Overlay histogram showing SH-SY5Y cells stained with ab90055 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90055, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab90055 has been referenced in 1 publication.
- Amaral LHP et al. ID Proteins May Reduce Aggressiveness of Thyroid Tumors. Endocr Pathol N/A:N/A (2018). PubMed: 30413933