Anti-ID2 antibody [OTI9A8] (ab236504)
Key features and details
- Mouse monoclonal [OTI9A8] to ID2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-ID2 antibody [OTI9A8]
See all ID2 primary antibodies -
Description
Mouse monoclonal [OTI9A8] to ID2 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Cynomolgus monkey, Orangutan -
Immunogen
Recombinant full length protein corresponding to Human ID2 aa 1 to the C-terminus. (NP_002157) produced in E.coli.
Database link: Q02363 -
Positive control
- WB: HEK-293T transfected with pCMV6-ENTRY ID2 whole cell lysate. IHC-P: Human liver carcinoma tissue. ICC/IF: COS-7 cells transiently transfected with pCMV6-ENTRY ID2.
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General notes
Clone OTI9A8 (formerly 9A8).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI9A8 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50.
Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes |
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WB |
1/500. Predicted molecular weight: 14 kDa.
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ICC/IF |
1/100.
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Notes |
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IHC-P
1/50. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes |
WB
1/500. Predicted molecular weight: 14 kDa. |
ICC/IF
1/100. |
Target
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Function
ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression. -
Tissue specificity
Highly expressed in early fetal tissues, including those of the central nervous system. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Developmental stage
Found in most early fetal tissues but not in the corresponding mature tissues. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 505025 Cow
- Entrez Gene: 3398 Human
- Entrez Gene: 15902 Mouse
- Entrez Gene: 654298 Pig
- Entrez Gene: 25587 Rat
- Omim: 600386 Human
- SwissProt: Q3ZC46 Cow
- SwissProt: Q4R5J7 Cynomolgus monkey
see all -
Alternative names
- bHLHb26 antibody
- Cell growth inhibiting gene 8 antibody
- class B basic helix loop helix protein 26 antibody
see all
Images
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All lanes : Anti-ID2 antibody [OTI9A8] (ab236504) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control, cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY ID2, cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 14 kDa -
Paraffin-embedded human liver carcinoma tissue stained for ID2 using ab236504 at 1/50 dilution in immunohistochemical analysis.
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COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY ID2 stained for ID2 (green) using ab236504 at 1/100 dilution in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236504 has not yet been referenced specifically in any publications.