Key features and details
- Mouse monoclonal [OTI9A8] to ID2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-ID2 antibody [OTI9A8]
See all ID2 primary antibodies
DescriptionMouse monoclonal [OTI9A8] to ID2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Cynomolgus monkey, Orangutan
- WB: HEK-293T transfected with pCMV6-ENTRY ID2 whole cell lysate. IHC-P: Human liver carcinoma tissue. ICC/IF: COS-7 cells transiently transfected with pCMV6-ENTRY ID2.
Clone OTI9A8 (formerly 9A8).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes
|WB||1/500. Predicted molecular weight: 14 kDa.|
FunctionID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression.
Tissue specificityHighly expressed in early fetal tissues, including those of the central nervous system.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Developmental stageFound in most early fetal tissues but not in the corresponding mature tissues.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- bHLHb26 antibody
- Cell growth inhibiting gene 8 antibody
- class B basic helix loop helix protein 26 antibody
All lanes : Anti-ID2 antibody [OTI9A8] (ab236504) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control, cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY ID2, cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 14 kDa
Paraffin-embedded human liver carcinoma tissue stained for ID2 using ab236504 at 1/50 dilution in immunohistochemical analysis.
COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY ID2 stained for ID2 (green) using ab236504 at 1/100 dilution in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236504 has not yet been referenced specifically in any publications.