Product nameID2 overexpression 293T lysate (whole cell)
DescriptionID2 overexpression lysate
ab94214 is a 293T cell transfected lysate in which Human ID2 has been transiently over-expressed using a pCMV-ID2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol
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BackgroundDevelopmental stage: Found in most early fetal tissues but not in the corresponding mature tissues. Function: ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-2 may be an inhibitor of tissue-specific gene expression. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. Tissue specificity: Highly expressed in early fetal tissues, including those of the central nervous system.
Our Abpromise guarantee covers the use of ab94214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94214 at 15μg/lane on an SDS-PAGE gel.
All lanes : Anti-ID2 antibody (ab89530) at 1/500 dilution
Lane 1 :
ID2 overexpression 293T lysate (whole cell) (ab94214)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution
ab94214 has not yet been referenced specifically in any publications.