Overview

  • Product name

  • Description

    Rabbit polyclonal to ID3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human ID3 (C terminal).

  • Positive control

    • Human breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab41834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 20460439
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.
  • Tissue specificity

    Expressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1R21 antibody
    • bHLHb25 antibody
    • Class B basic helix-loop-helix protein 25 antibody
    • DNA binding protein inhibitor ID 3 antibody
    • DNA-binding protein inhibitor ID-3 antibody
    • HEIR 1 antibody
    • HEIR1 antibody
    • Helix loop helix protein HEIR 1 antibody
    • Helix loop helix protein HEIR1 antibody
    • Helix-loop-helix protein HEIR-1 antibody
    • HLH642 antibody
    • ID 3 antibody
    • ID like protein inhibitor HLH 1R21 antibody
    • ID like protein inhibitor HLH 462 antibody
    • ID-like protein inhibitor HLH 1R21 antibody
    • ID3 antibody
    • ID3_HUMAN antibody
    • Inhibitor of DNA binding 3 antibody
    • Inhibitor of DNA binding 3 dominant negative helix loop helix protein antibody
    see all

Images

  • Ab41834 staining human ID3 in human breast carcinoma by immunohistochemistry using formalin fixed, paraffin embedded tissue.
  • ICC/IF image of ab41834 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41834, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Xu C  et al. The microRNA-10a/ID3/RUNX2 axis modulates the development of Ossification of Posterior Longitudinal Ligament. Sci Rep 8:9225 (2018). Read more (PubMed: 29907859) »
  • Forni PE  et al. The indirect role of fibroblast growth factor-8 in defining neurogenic niches of the olfactory/GnRH systems. J Neurosci 33:19620-34 (2013). Mouse . Read more (PubMed: 24336726) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of alternative antibody ab41834 with the order number ####.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (breast cancer, prostate cancer, kidney)
Specification
breast cancer, prostate cancer, kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, 20 minutes at 100C
Permeabilization
No

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Verified customer

Submitted Feb 17 2009

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