Product nameAnti-ID3 antibody
See all ID3 primary antibodies
DescriptionRabbit polyclonal to ID3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
3 synthetic peptides (Human) conjugated to KLH
- Recombinant protein produced in an induced culture of E.coli.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab86017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 13 kDa.|
FunctionID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.
Tissue specificityExpressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
- Information by UniProt
- 1R21 antibody
- bHLHb25 antibody
- Class B basic helix-loop-helix protein 25 antibody
All lanes : Anti-ID3 antibody (ab86017) at 1/1000 dilution
Lane 1 : Uninduced (negative control) culture of E.coli
Lane 2 : Induced culture of E.coli
All lanes : Anti-Rabbit IgG HRP conjugated at 1/1000 dilution
Predicted band size: 13 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Additional bands at: 124 kDa, 209 kDa, 32 kDa, 45 kDa, 75 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 10%Predicted molecular weight of recombinant ID3:27kDa (13kDa + another 14kDa for the tag).
ab86017 has not yet been referenced specifically in any publications.