Key features and details
- Mouse monoclonal [OTI8B3] to ID3
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-ID3 antibody [OTI8B3]
See all ID3 primary antibodies
DescriptionMouse monoclonal [OTI8B3] to ID3
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
- WB: HEK-293T transfected with pCMV6-ENTRY ID3 cDNA, whole cell lysate. IHC-P: Human colon, kidney, liver, ovary, thyroid and prostate tissue. ICC/IF: COS-7 cells transiently transfected with pCMV6-ENTRY ID3 cDNA. Flow: HeLa and Jurkat cells.
Clone OTI8B3 (formerly 8B3).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
|WB||1/500. Predicted molecular weight: 13 kDa.|
FunctionID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID-3 inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors.
Tissue specificityExpressed abundantly in lung, kidney and adrenal gland, but not in adult brain.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
- Information by UniProt
- 1R21 antibody
- bHLHb25 antibody
- Class B basic helix-loop-helix protein 25 antibody
All lanes : Anti-ID3 antibody [OTI8B3] (ab236505) at 1/500 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control cDNA, whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY ID3 cDNA, whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 13 kDa
Paraffin-embedded human colon tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human ovary tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human thyroid tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human prostate tissue stained for ID3 using ab236505 at 1/50 dilution in immunohistochemical analysis.
COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY ID3 cDNA stained for ID3 (green) using ab236505 at 1/100 dilution in ICC/IF.
Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling ID3 with ab236505 at 1/100 dilution (red) compared to a negative control antibody (blue).
Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling ID3 with ab236505 at 1/100 dilution (red) compared to a negative control antibody (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236505 has not yet been referenced specifically in any publications.