Overview

  • Product name

  • Description

    Rabbit polyclonal to ID4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig
  • Immunogen

    A reigon within synthetic peptide: MKAVSPVRPS GRKAPSGCGG GELALRCLAE HGHSLGGSAA AAAAAAAARC, corresponding to N terminal amino acids 1-50 of Human ID4

  • Positive control

    • HepG2 whole cell lysate (ab7900)

Properties

Applications

Our Abpromise guarantee covers the use of ab49261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 18 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration. PubMed: 21297112

Target

Images

  • Anti-ID4 antibody (ab49261) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG 1/50000-1/100000

    Predicted band size: 18 kDa
    Observed band size: 18 kDa

References

This product has been referenced in:

  • Jeon HY  et al. Ly6G+ inflammatory cells enable the conversion of cancer cells to cancer stem cells in an irradiated glioblastoma model. Cell Death Differ N/A:N/A (2019). Read more (PubMed: 30804471) »
  • Amaral LHP  et al. ID Proteins May Reduce Aggressiveness of Thyroid Tumors. Endocr Pathol N/A:N/A (2018). Read more (PubMed: 30413933) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, postnatal day 6)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, postnatal day 6
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Mr. Bryan Niedenberger

Verified customer

Submitted Sep 15 2016

Abreviews
Application
Western blot
Sample
Rat Tissue lysate - other (spinal cord)
Loading amount
10 µg
Specification
spinal cord
Gel Running Conditions
Reduced Denaturing (10%gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Ms. Minjung Kwon

Verified customer

Submitted Jan 21 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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