Overview

  • Product name

  • Description

    Rabbit polyclonal to IDH1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human IDH1 aa 116-143 (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: O75874

  • Positive control

    • HepG2 and 293 cells; Human hepatocarcinoma tissue; HepG2 whole cell lysate (ab7900); Mouse liver tissue lysates.
  • General notes

     This product was previously labelled as Isocitrate dehydrogenase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab135659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
WB 1/50 - 1/100. Predicted molecular weight: 46 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Involvement in disease

    Glioma
    Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
  • Sequence similarities

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.
  • Post-translational
    modifications

    Acetylation at Lys-374 dramatically reduces catalytic activity.
  • Cellular localization

    Cytoplasm. Peroxisome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytosolic NADP isocitrate dehydrogenase antibody
    • Cytosolic NADP-isocitrate dehydrogenase antibody
    • Epididymis luminal protein 216 antibody
    • Epididymis secretory protein Li 26 antibody
    • HEL-216 antibody
    • HEL-S-26 antibody
    • ICDH antibody
    • IDCD antibody
    • IDH antibody
    • IDH1 antibody
    • IDHC_HUMAN antibody
    • IDP antibody
    • IDPC antibody
    • Isocitrate dehydrogenase (NADP(+)) 1 cytosolic antibody
    • Isocitrate dehydrogenase [NADP] cytoplasmic antibody
    • Isocitrate dehydrogenase 1 (NADP+) soluble antibody
    • NADP dependent isocitrate dehydrogenase cytosolic antibody
    • NADP dependent isocitrate dehydrogenase peroxisomal antibody
    • NADP(+)-specific ICDH antibody
    • Oxalosuccinate decarboxylase antibody
    • PICD antibody
    see all

Images

  • All lanes : Anti-IDH1 antibody (ab135659) at 1/50 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Mouse liver tissue lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 46 kDa

  • Immunoflouorescent analysis of HepG2 cells labelling IDH1 with ab135659 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI to stain the cell nuclear (blue).

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocarcinoma tissue labelling IDH1 with ab135659 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Flow cytometric analysis of 293 cells labelling Isocitrate-dehydrogenase with ab135659 at 1/10 dilution (green) or negative control cell (blue), using FITC-conjugated goat anti-rabbit secondary antibodies.

References

This product has been referenced in:

  • Gowda P  et al. Mutant Isocitrate Dehydrogenase 1 Disrupts PKM2-ß-Catenin-BRG1 Transcriptional Network-Driven CD47 Expression. Mol Cell Biol 38:N/A (2018). Read more (PubMed: 29463646) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

I am assuming the species customer of tissue sections is human - antibodeis are asfollows;

The best anti annexin a1 antibody is ab47661;https://www.abcam.com/Annexin-A1-antibody-ab47661.html

anti IDH1 ab135659; https://www.abcam.com/Isocitrate-dehydrogenase-antibody-ab135659.html

anti MDM2, ab38618; https://www.abcam.com/MDM2-antibody-ab38618.html

anti PMS2, ab111253; https://www.abcam.com/PMS2-antibody-ab111253.html

ab9144; https://www.abcam.com/MLH1-antibody-ab9144.html

MSH2, ab16833; https://www.abcam.com/MSH2-antibody-ab16833.html

MSH6, ab137457; https://www.abcam.com/MSH6-antibody-ab137457.html

anti Napsin, ab73098; https://www.abcam.com/NAPSIN-A-antibody-KCG1-1-prediluted-ab73098.html

Caldesmon, ab68878; https://www.abcam.com/Caldesmon-antibody-ab68878.html

p16????? ab118459; https://www.abcam.com/p16-ARC-antibody-ab118459.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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