• Product name

  • Description

    Rabbit polyclonal to IDH1
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human IDH1 aa 116-143 (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: O75874

  • Positive control

    • HepG2 and 293 cells; Human hepatocarcinoma tissue; HepG2 whole cell lysate (ab7900); Mouse liver tissue lysates.
  • General notes

     This product was previously labelled as Isocitrate dehydrogenase




Our Abpromise guarantee covers the use of ab135659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
WB 1/50 - 1/100. Predicted molecular weight: 46 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.



  • Involvement in disease

    Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
  • Sequence similarities

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.
  • Post-translational

    Acetylation at Lys-374 dramatically reduces catalytic activity.
  • Cellular localization

    Cytoplasm. Peroxisome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytosolic NADP isocitrate dehydrogenase antibody
    • Cytosolic NADP-isocitrate dehydrogenase antibody
    • Epididymis luminal protein 216 antibody
    • Epididymis secretory protein Li 26 antibody
    • HEL-216 antibody
    • HEL-S-26 antibody
    • ICDH antibody
    • IDCD antibody
    • IDH antibody
    • IDH1 antibody
    • IDHC_HUMAN antibody
    • IDP antibody
    • IDPC antibody
    • Isocitrate dehydrogenase (NADP(+)) 1 cytosolic antibody
    • Isocitrate dehydrogenase [NADP] cytoplasmic antibody
    • Isocitrate dehydrogenase 1 (NADP+) soluble antibody
    • NADP dependent isocitrate dehydrogenase cytosolic antibody
    • NADP dependent isocitrate dehydrogenase peroxisomal antibody
    • NADP(+)-specific ICDH antibody
    • Oxalosuccinate decarboxylase antibody
    • PICD antibody
    see all


  • All lanes : Anti-IDH1 antibody (ab135659) at 1/50 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Mouse liver tissue lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 46 kDa

  • Immunoflouorescent analysis of HepG2 cells labelling IDH1 with ab135659 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI to stain the cell nuclear (blue).

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocarcinoma tissue labelling IDH1 with ab135659 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Flow cytometric analysis of 293 cells labelling Isocitrate-dehydrogenase with ab135659 at 1/10 dilution (green) or negative control cell (blue), using FITC-conjugated goat anti-rabbit secondary antibodies.


This product has been referenced in:

  • Gowda P  et al. Mutant Isocitrate Dehydrogenase 1 Disrupts PKM2-ß-Catenin-BRG1 Transcriptional Network-Driven CD47 Expression. Mol Cell Biol 38:N/A (2018). Read more (PubMed: 29463646) »
See 1 Publication for this product

Customer reviews and Q&As


Thank you for contacting us.

I am assuming the species customer of tissue sections is human - antibodeis are asfollows;

The best anti annexin a1 antibody is ab47661;https://www.abcam.com/Annexin-A1-antibody-ab47661.html

anti IDH1 ab135659; https://www.abcam.com/Isocitrate-dehydrogenase-antibody-ab135659.html

anti MDM2, ab38618; https://www.abcam.com/MDM2-antibody-ab38618.html

anti PMS2, ab111253; https://www.abcam.com/PMS2-antibody-ab111253.html

ab9144; https://www.abcam.com/MLH1-antibody-ab9144.html

MSH2, ab16833; https://www.abcam.com/MSH2-antibody-ab16833.html

MSH6, ab137457; https://www.abcam.com/MSH6-antibody-ab137457.html

anti Napsin, ab73098; https://www.abcam.com/NAPSIN-A-antibody-KCG1-1-prediluted-ab73098.html

Caldesmon, ab68878; https://www.abcam.com/Caldesmon-antibody-ab68878.html

p16????? ab118459; https://www.abcam.com/p16-ARC-antibody-ab118459.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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