Key features and details
- Rabbit polyclonal to IDH1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Product nameAnti-IDH1 antibody
See all IDH1 primary antibodies
DescriptionRabbit polyclonal to IDH1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human, Pig
Predicted to work with: Rat, Sheep, Cow, Orangutan, Prairie vole
Recombinant fragment (His-tag) corresponding to Human IDH1 aa 74-333. N-Terminal tag. (Expressed in E.coli).
ATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSG WVKPIIIGRHAYGDQYRATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFE EGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLSTKNTILKKYDGRF KDIFQEIYDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYD GDVQSDSVAQGYGSLGMMTSVLVCPDGKTVEAEAAHGTVTRHYRMYQKGQ ETSTNPIASI
Database link: O75874
- IHC-P: Human liver tissue. WB: Recombinant human IDH1 protein; Mouse, pig and human liver lysate; HepG2 cell lysate.
This product was previously labelled as Isocitrate dehydrogenase
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab233202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 46 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
Involvement in diseaseGlioma
Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
Sequence similaritiesBelongs to the isocitrate and isopropylmalate dehydrogenases family.
modificationsAcetylation at Lys-374 dramatically reduces catalytic activity.
Cellular localizationCytoplasm. Peroxisome.
- Information by UniProt
- Cytosolic NADP isocitrate dehydrogenase antibody
- Cytosolic NADP-isocitrate dehydrogenase antibody
- Epididymis luminal protein 216 antibody
All lanes : Anti-IDH1 antibody (ab233202) at 2 µg/ml
Lane 1 : Mouse liver tissue lysate.
Lane 2 : Human liver tissue lysate.
Lane 3 : Pig liver tissue lysate.
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate.
Predicted band size: 46 kDa
Anti-IDH1 antibody (ab233202) at 2 µg/ml + Recombinant human IDH1 protein.
Predicted band size: 46 kDa
Formalin-fixed, paraffin-embedded human liver tissue stained for IDH1 using ab233202 at 20µg/mL in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233202 has not yet been referenced specifically in any publications.