Overview

  • Product name

  • Description

    Rabbit polyclonal to IDH1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide within Human IDH1 aa 364-414. The exact sequence is proprietary. NP_005887.2
    Database link: O75874

  • Positive control

    • WB: K562, HeLa, HEK-293T, NIH/3T3 and C6 whole cell lysates. IP: Jurkat whole cell lysate.
  • General notes

     This product was previously labelled as Isocitrate dehydrogenase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab245320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/500 - 1/2500.

Target

  • Involvement in disease

    Glioma
    Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
  • Sequence similarities

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.
  • Post-translational
    modifications

    Acetylation at Lys-374 dramatically reduces catalytic activity.
  • Cellular localization

    Cytoplasm. Peroxisome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytosolic NADP isocitrate dehydrogenase antibody
    • Cytosolic NADP-isocitrate dehydrogenase antibody
    • Epididymis luminal protein 216 antibody
    • Epididymis secretory protein Li 26 antibody
    • HEL-216 antibody
    • HEL-S-26 antibody
    • ICDH antibody
    • IDCD antibody
    • IDH antibody
    • IDH1 antibody
    • IDHC_HUMAN antibody
    • IDP antibody
    • IDPC antibody
    • Isocitrate dehydrogenase (NADP(+)) 1 cytosolic antibody
    • Isocitrate dehydrogenase [NADP] cytoplasmic antibody
    • Isocitrate dehydrogenase 1 (NADP+) soluble antibody
    • NADP dependent isocitrate dehydrogenase cytosolic antibody
    • NADP dependent isocitrate dehydrogenase peroxisomal antibody
    • NADP(+)-specific ICDH antibody
    • Oxalosuccinate decarboxylase antibody
    • PICD antibody
    see all

Images

  • All lanes : Anti-IDH1 antibody (ab245320) at 1 µg

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
    Lane 6 : C6 (rat glioma cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Exposure time: 30 seconds
  • IDH1 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245320 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245320 at 1 µg/ml.

    Lane 1: ab245320 IP in Jurkat whole cell lysate.
    Lane 2: Control IgG IP in Jurkat whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab245320 has not yet been referenced specifically in any publications.

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