Key features and details
- Rabbit polyclonal to IDH1
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-IDH1 antibody
See all IDH1 primary antibodies
DescriptionRabbit polyclonal to IDH1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Orangutan
Synthetic peptide within Human IDH1 aa 364-414. The exact sequence is proprietary. NP_005887.2
Database link: O75874
- WB: K562, HeLa, HEK-293T, NIH/3T3 and C6 whole cell lysates. IP: Jurkat whole cell lysate.
This product was previously labelled as Isocitrate dehydrogenase
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245320 was affinity purified using an epitope specific to IDH1 immobilized on solid support.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab245320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/500 - 1/2500.|
Involvement in diseaseGlioma
Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
Sequence similaritiesBelongs to the isocitrate and isopropylmalate dehydrogenases family.
modificationsAcetylation at Lys-374 dramatically reduces catalytic activity.
Cellular localizationCytoplasm. Peroxisome.
- Information by UniProt
- Cytosolic NADP isocitrate dehydrogenase antibody
- Cytosolic NADP-isocitrate dehydrogenase antibody
- Epididymis luminal protein 216 antibody
All lanes : Anti-IDH1 antibody (ab245320) at 1 µg
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 6 : C6 (rat glioma cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 seconds
IDH1 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245320 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245320 at 1 µg/ml.
Lane 1: ab245320 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245320 has not yet been referenced specifically in any publications.