Overview

  • Product name

  • Description

    Rabbit polyclonal to IDH1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Caenorhabditis elegans, Drosophila melanogaster
  • Immunogen

    Synthetic peptide corresponding to Human IDH1 aa 288-337 (internal sequence).
    Database link: NP_005887

  • Positive control

    • 721_B cell lysate
  • General notes

     This product was previously labelled as Isocitrate dehydrogenase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab81653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.

Target

Images

  • Rabbit Anti-IDH1 Antibody ab81653. Paraffin Embedded Tissue: Human Testis. Antibody Concentration: 5 ug/ml
  • Anti-IDH1 antibody (ab81653) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa
    Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.

  • Sample Type : Human glioma cells. Primary Antibody Dilution : 1:200. Secondary Antibody : Anti-rabbit-GFP. Secondary Antibody Dilution : 1:5000. Color/Signal Descriptions : 1. Red: Nucleus 2. Green: IDH 3. Merge. Gene Name : IDH1.
  • ab81653 at 5 µg/ml staining IDH1 in Human kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  • ab81653 at 5 µg/ml staining IDH1 in Human testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

ab81653 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Drosophila melanogaster Cell (Drosophila Embryos)
Permeabilization
Yes - Hexane
Specification
Drosophila Embryos
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde

Ben DiCiaccio

Verified customer

Submitted Jan 18 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (Prostate cancer cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Prostate cancer cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 30 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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