Overview

  • Product name

  • Description

    Rabbit polyclonal to IDH1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Pig
    Predicted to work with: Mouse
  • Immunogen

    Full length native protein (purified) corresponding to Pig IDH1.

  • Positive control

    • Heart tissue.
  • General notes

     This product was previously labelled as Isocitrate dehydrogenase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab85532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
ELISA 1/400 - 1/1600.
IHC-P Use a concentration of 10 µg/ml.

Target

  • Involvement in disease

    Glioma
    Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
  • Sequence similarities

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.
  • Post-translational
    modifications

    Acetylation at Lys-374 dramatically reduces catalytic activity.
  • Cellular localization

    Cytoplasm. Peroxisome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytosolic NADP isocitrate dehydrogenase antibody
    • Cytosolic NADP-isocitrate dehydrogenase antibody
    • Epididymis luminal protein 216 antibody
    • Epididymis secretory protein Li 26 antibody
    • HEL-216 antibody
    • HEL-S-26 antibody
    • ICDH antibody
    • IDCD antibody
    • IDH antibody
    • IDH1 antibody
    • IDHC_HUMAN antibody
    • IDP antibody
    • IDPC antibody
    • Isocitrate dehydrogenase (NADP(+)) 1 cytosolic antibody
    • Isocitrate dehydrogenase [NADP] cytoplasmic antibody
    • Isocitrate dehydrogenase 1 (NADP+) soluble antibody
    • NADP dependent isocitrate dehydrogenase cytosolic antibody
    • NADP dependent isocitrate dehydrogenase peroxisomal antibody
    • NADP(+)-specific ICDH antibody
    • Oxalosuccinate decarboxylase antibody
    • PICD antibody
    see all

Images

  • ab85532 at 10µg/ml staining IDH1 in human heart tissue, by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue

References

ab85532 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.

As I understand it, if the recommended IHC working concentration for this antibody is 10ug/ml, and 100ul are needed for slide, then you would need 1ug of antibody per 100ul of diluent, that is, 1ug of antibody per slide.

As 50ug of antibody you would then have enough antibody to stain 50 slides, if I am not mistaken.

Regarding antibody ab117976, it is not a better antibody, it’s a different one against the same target. The main differences between both are the immunogen used to produce it and the clonality. Ab117976 is a Mouse monoclonal, whereas ab85532 is a Rabbit polyclonal.

If you want to have a look at the advantages and disadvantages of using polyclonal and monoclonal, you can have a look at our protocol’s site:

https://www.abcam.com/index.html?pageconfig=resource&rid=11269&pid=11287

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us. I would say that this sort of experiment is very-very tricky. We unfortunately do not have any protocol available at the moment for manually IHC staining quantification with antibodies. I would suggest ploughing through the publications and finding if there are scientists who are using similar kind of method. http://www.histochem.org/archives/photoshop.pdf http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1810239/ http://www.ncbi.nlm.nih.gov/pubmed/21370029 Have a look at these articles and check which one could help you to set up the experiment. I hope this will help.

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Question
Answer

Thank you for your enquiry. This antibody was raised against isocytrate dehydrogenase purified from porcine heart. According to our collaborator who developed the antibody, they have only tested it against wild type isocytrate dehydrogenase. It has not been tested against any mutant forms of the protein. Therefore, we do not know if it could also detect the mutant form. Please note that this antibody is thought to recognize both the cytosolic and the mitochondrial form of the protein. Also, the tissue shown in the image is from IHC using FFPE human heart.

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