Overview

  • Product name

  • Description

    Rabbit polyclonal to IDH1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow
  • Immunogen

    Synthetic peptide corresponding to Human IDH1 aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab105207)

  • Positive control

    • This antibody gave a positive signal in HepG2 whole cell lysate as well as the following tissue lysates: Human kidney; Human testis. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.
  • General notes

     This product was previously labelled as Isocitrate dehydrogenase

     

Properties

Applications

Our Abpromise guarantee covers the use of ab94571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Involvement in disease

    Glioma
    Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
  • Sequence similarities

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.
  • Post-translational
    modifications

    Acetylation at Lys-374 dramatically reduces catalytic activity.
  • Cellular localization

    Cytoplasm. Peroxisome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytosolic NADP isocitrate dehydrogenase antibody
    • Cytosolic NADP-isocitrate dehydrogenase antibody
    • Epididymis luminal protein 216 antibody
    • Epididymis secretory protein Li 26 antibody
    • HEL-216 antibody
    • HEL-S-26 antibody
    • ICDH antibody
    • IDCD antibody
    • IDH antibody
    • IDH1 antibody
    • IDHC_HUMAN antibody
    • IDP antibody
    • IDPC antibody
    • Isocitrate dehydrogenase (NADP(+)) 1 cytosolic antibody
    • Isocitrate dehydrogenase [NADP] cytoplasmic antibody
    • Isocitrate dehydrogenase 1 (NADP+) soluble antibody
    • NADP dependent isocitrate dehydrogenase cytosolic antibody
    • NADP dependent isocitrate dehydrogenase peroxisomal antibody
    • NADP(+)-specific ICDH antibody
    • Oxalosuccinate decarboxylase antibody
    • PICD antibody
    see all

Images

  • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: IDH1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab94571 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab94571 was shown to specifically recognize IDH1 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when IDH1 knockout samples were examined. Wild-type and IDH1 knockout samples were subjected to SDS-PAGE.  Ab94571 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-IDH1 antibody (ab94571) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa
    Additional bands at: 28 kDa, 78 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • ICC/IF image of ab94571 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab94571, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa cells at 5µg/ml.
  • IHC image of IDH1 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94571, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Lee WD  et al. Spatial-fluxomics provides a subcellular-compartmentalized view of reductive glutamine metabolism in cancer cells. Nat Commun 10:1351 (2019). Read more (PubMed: 30903027) »
  • Niittykoski M  et al. Immunohistochemical Characterization and Sensitivity to Human Adenovirus Serotypes 3, 5, and 11p of New Cell Lines Derived from Human Diffuse Grade II to IV Gliomas. Transl Oncol 10:772-779 (2017). Read more (PubMed: 28797937) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Pas de problème, j'ai donc demandé à notre service comptabilité de mettre en place un avoir, référence CN est ****** (pour le ab94571). Voici les manières dont vous pouvez utiliser cette référence CN: (1) la faire valoir contre la facture originale si celle-ci n'as pas encore été payée (2) la faire valoir contre une prochaine facture (3) obtenir un remboursement de la somme entière si vous n'avez pas de factures actuellement en cours avec Abcam Si vous souhaitez recevoir un remboursement au lieu d'un avoir, veuillez demander à votre service de gestion de prendre contact avec notre département comptabilité afin que nous puissions recueillir les informations nécessaires pour cette restitution. Notre service comptabilité peut être contacté par courriel à l'adresse creditcontrol@abcam.com ou par téléphone en utilisant le lien « Contactez-nous » dans le coin en haut à droite de notre site internet. Veuillez communiquer votre numéro d'avoir lorsque vous contactez notre service comptabilité. Vous recevrez également la référence complète de la note de crédit par courriel ou par voie postale; cette référence commençant par les lettres CGB. Concernant l'anti-galectine 1 (ab25138), le numéro de commande de remplacement gratuit de ce produit par uine unité de ab58085 est ********. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition. L'équipe technique reste à votre disposition pour plus de conseils. Bonne continuation dans votre recherche.

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Answer

Merci pour ces informations. Je suis désolé d'apprendre qu'aucun des 2 anticorps de remplacement n'a fonctionné en western blot sur échantillons humains. Pour des raisons de qualité, j'ai transmis vos données et commentaires au laboratoire afin que des tests supplémentaires soient entrepris pour ces 2 références. Afin de résoudre ce problème le plus rapidement possible, pourriez-vous m'indiquer si vous préférez recevoir un avoir ou un remboursement contre la commande originale ou un remplacement avec des anticorps différents? Pour la détection de la Galectine 1 humaine en western blot, nous avons les 5 anticorps suivants (testés WB et humain) : • ab108389, rabbit monoclonal, www.abcam.com/ab108389 • ab38328, rabbit polyclonal, www.abcam.com/ab38328 • ab114087, goat polyclonal, www.abcam.com/ab114087 • ab58085, mouse monoclonal, www.abcam.com/ab58085 • ab112525, rabbit polyclonal, www.abcam.com/ab112525 Pour la détection de la isocitrate deshydrogenase humaine en western blot, nous avons les 5 anticorps suivants (testés WB et humain) : • ab117976, mouse monoclonal, www.abcam.com/ab117976 • ab113232, rabbit polyclonal, www.abcam.com/ab113232 • ab81653, rabbit polyclonal, www.abcam.com/ab81653 • ab85532, rabbit polyclonal, www.abcam.com/ab85532 • ab117539, rabbit polyclonal, www.abcam.com/ab117539 Merci de me confirmer la résolution qui vous conviendra le mieux.

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