Recombinant Anti-IDH1 antibody [EPR12296] (ab172964)
![Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)](https://www.abcam.com/ps/products/172/ab172964/Images/ab172964-467538-anti-idh1-antibody-epr12296-westernblot-raji-human.jpg "Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12296] to IDH1
- Suitable for: WB, IHC-P, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-IDH1 antibody [EPR12296]
See all IDH1 primary antibodies -
Description
Rabbit monoclonal [EPR12296] to IDH1 -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
-
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Cow, Orangutan
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HAP1, HepG2, and HeLa cell lysates. IHC-P: Human gastric cancer tissue IP: HepG2 whole cell lysate WB: Raji, HepG2 and Neuro-2a whole cell lysates. Rat kidney lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12296 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Assay kits
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
-
Substrate reagent
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
|
| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 47 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
|
IP
1/10 - 1/100. |
Target
-
Involvement in disease
Glioma
Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. -
Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
Post-translational
modificationsAcetylation at Lys-374 dramatically reduces catalytic activity. -
Cellular localization
Cytoplasm. Peroxisome. - Information by UniProt
-
Database links
- Entrez Gene: 281235 Cow
- Entrez Gene: 3417 Human
- Entrez Gene: 15926 Mouse
- Entrez Gene: 100171634 Orangutan
- Entrez Gene: 24479 Rat
- Entrez Gene: 443257 Sheep
- Omim: 147700 Human
- SwissProt: Q9XSG3 Cow
see all -
Alternative names
- Cytosolic NADP isocitrate dehydrogenase antibody
- Cytosolic NADP-isocitrate dehydrogenase antibody
- Epididymis luminal protein 216 antibody
see all
Images
-
Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)Anti-IDH1 antibody [EPR12296] (ab172964) at 1/1000 dilution (Purified) + Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 47 kDa -
![Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)](https://www.abcam.com/ps/products/172/ab172964/Images/ab172964-467537-anti-idh1-antibody-epr12296-westernblot-human-mouse-rat.jpg)
Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)All lanes : Anti-IDH1 antibody [EPR12296] (ab172964) at 1/1000 dilution (Purified)
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : Rat kidney lysate
Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 47 kDa -
![Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)](https://www.abcam.com/ps/products/172/ab172964/Images/ab172964-280457-anti-isocitrate-dehydrogenase-antibody-epr12296-western-blot.jpg)
Western blot - Anti-IDH1 antibody [EPR12296] (ab172964)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: IDH1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab172964 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab172964 was shown to specifically react with IDH1 in wild-type HAP1 cells. No band was observed when IDH1 knockout samples were used. Wild-type and IDH1 knockout samples were subjected to SDS-PAGE. ab172964 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [EPR12296] (ab172964)](https://www.abcam.com/ps/products/172/ab172964/Images/ab172964-6-anti-idh1-antibody-epr12296-immunohistochemistry-gastric-cancer-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH1 antibody [EPR12296] (ab172964)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric cancer tissue sections labeling IDH1 with purified ab172964 at 1/200 dilution (0.60 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. -
![Immunoprecipitation - Anti-IDH1 antibody [EPR12296] (ab172964)](https://www.abcam.com/ps/products/172/ab172964/Images/ab172964-7-anti-idh1-antibody-epr12296-immunoprecipitation-hepg2-human.jpg)
Immunoprecipitation - Anti-IDH1 antibody [EPR12296] (ab172964)Purified ab172964 at 1/20 dilution (0.6µg) immunoprecipitating IDH1 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate.
Lane 2 (+): ab172964 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab172964 in HepG2 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST. -
![Anti-IDH1 antibody [EPR12296] (ab172964)](https://www.abcam.com/ps/products/172/ab172964/Images/ab172964-5-benefits-of-recombinant-antibodies.png)
Anti-IDH1 antibody [EPR12296] (ab172964)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (9)
ab172964 has been referenced in 9 publications.
- Zhang M et al. The Inhibition of B7H3 by 2-HG Accumulation Is Associated With Downregulation of VEGFA in IDH Mutated Gliomas. Front Cell Dev Biol 9:670145 (2021). PubMed: 34079802
- Pillai-Kastoori L et al. Antibody validation for Western blot: By the user, for the user. J Biol Chem 295:926-939 (2020). PubMed: 31819006
- Shi F et al. Wild-type IDH2 contributes to Epstein-Barr virus-dependent metabolic alterations and tumorigenesis. Mol Metab 36:100966 (2020). PubMed: 32224436
- Wei X et al. Reducing NADPH Synthesis Counteracts Diabetic Nephropathy through Restoration of AMPK Activity in Type 1 Diabetic Rats. Cell Rep 32:108207 (2020). PubMed: 32997989
- Nie Y et al. Lactobacillus frumenti improves antioxidant capacity via nitric oxide synthase 1 in intestinal epithelial cells. FASEB J 33:10705-10716 (2019). PubMed: 31262191
- Liu Z et al. High expression of PIM2 induces HSC proliferation in myelodysplastic syndromes via the IDH1/HIF1-a signaling pathway. Oncol Lett 17:5395-5402 (2019). PubMed: 31186757
- Zhang W et al. Ketamine exerts a protective role in a cell-based model of major depressive disorder via the inhibition of apoptosis and inflammation and activation of the Krebs cycle. Bosn J Basic Med Sci N/A:N/A (2019). PubMed: 31215856
- Costa Nunes F et al. Tacrine derivatives stimulate human glioma SF295 cell death and alter important proteins related to disease development: An old drug for new targets. Biochim Biophys Acta 1862:1527-1536 (2018). PubMed: 29704527
- Ma S et al. D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth. Oncotarget 6:8606-20 (2015). PubMed: 25825982