Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-IDH1 antibody [EPR12296] - BSA and Azide free (ab214803)

Overview

  • Product name

    Anti-IDH1 antibody [EPR12296] - BSA and Azide free
    See all IDH1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12296] to IDH1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Cow, Orangutan
  • Immunogen

    Synthetic peptide within Human IDH1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O75874

  • Positive control

    • HepG2, HeLa, Raji and fetal kidney lysates; Human breast carcinoma tissue.
  • General notes

    Ab214803 is the carrier-free version of ab172964. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab214803 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab214803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
IP 1/10 - 1/100.

Target

  • Involvement in disease

    Glioma
    Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
  • Sequence similarities

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.
  • Post-translational
    modifications

    Acetylation at Lys-374 dramatically reduces catalytic activity.
  • Cellular localization

    Cytoplasm. Peroxisome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytosolic NADP isocitrate dehydrogenase antibody
    • Cytosolic NADP-isocitrate dehydrogenase antibody
    • Epididymis luminal protein 216 antibody
    • Epididymis secretory protein Li 26 antibody
    • HEL-216 antibody
    • HEL-S-26 antibody
    • ICDH antibody
    • IDCD antibody
    • IDH antibody
    • IDH1 antibody
    • IDHC_HUMAN antibody
    • IDP antibody
    • IDPC antibody
    • Isocitrate dehydrogenase (NADP(+)) 1 cytosolic antibody
    • Isocitrate dehydrogenase [NADP] cytoplasmic antibody
    • Isocitrate dehydrogenase 1 (NADP+) soluble antibody
    • NADP dependent isocitrate dehydrogenase cytosolic antibody
    • NADP dependent isocitrate dehydrogenase peroxisomal antibody
    • NADP(+)-specific ICDH antibody
    • Oxalosuccinate decarboxylase antibody
    • PICD antibody
    see all

Images

  • This WB data was generated using the same anti-IDH1 antibody clone, EPR12296, in a different buffer formulation (cat# ab172964).

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: IDH1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab172964 observed at 46 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab172964 was shown to specifically react with IDH1 in wild-type HAP1 cells. No band was observed when IDH1 knockout samples were used. Wild-type and IDH1 knockout samples were subjected to SDS-PAGE.  Ab172964 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot analysis on immunoprecipitation pellet from (1) HepG2 cell lysate or (2) 1X PBS (negative control) using ab172964 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172964).

  • This IHC data was generated using the same anti-IDH1 antibody clone, EPR12296, in a different buffer formulation (cat# ab172964).

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling IDH1 with ab172964  at 1/100 dilution.

References

ab214803 has not yet been referenced specifically in any publications.

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