Key features and details
- Mouse monoclonal [IDH1/1152] to IDH1 - BSA and Azide free
- Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-IDH1 antibody [IDH1/1152] - BSA and Azide free
See all IDH1 primary antibodies
DescriptionMouse monoclonal [IDH1/1152] to IDH1 - BSA and Azide free
Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human IDH1 aa 281-414. The exact sequence is proprietary.
Database link: O75874
- HepG2, HeLa, HT29 or MCF7 cells; Breast, Colon or Prostate Carcinoma.
This product was previously labelled as Isocitrate dehydrogenase
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein G purified
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab212470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5-1µg for 106 cells.
Final volume 100 µL.
|ICC/IF||Use a concentration of 0.5 - 1 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 45-47 kDa (predicted molecular weight: 46 kDa).|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
Involvement in diseaseGlioma
Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
Sequence similaritiesBelongs to the isocitrate and isopropylmalate dehydrogenases family.
modificationsAcetylation at Lys-374 dramatically reduces catalytic activity.
Cellular localizationCytoplasm. Peroxisome.
- Information by UniProt
- Cytosolic NADP isocitrate dehydrogenase antibody
- Cytosolic NADP-isocitrate dehydrogenase antibody
- Epididymis luminal protein 216 antibody
ab212470 has not yet been referenced specifically in any publications.