Anti-IDH1 antibody - N-terminal (ab226190)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-IDH1 antibody - N-terminal
See all IDH1 primary antibodies -
Description
Rabbit polyclonal to IDH1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Dog, Pig, Chimpanzee, Orangutan
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Immunogen
Synthetic peptide within Human IDH1 aa 1-50 (N terminal). The exact sequence is proprietary. (NP_005887.2).
Database link: O75874 -
Positive control
- IP: Jurkat whole cell lysate.
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General notes
This product was previously labelled as Isocitrate dehydrogenase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226190 was affinity purified using an epitope specific to IDH1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab226190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Involvement in disease
Glioma
Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. -
Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
Post-translational
modificationsAcetylation at Lys-374 dramatically reduces catalytic activity. -
Cellular localization
Cytoplasm. Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 460113 Chimpanzee
- Entrez Gene: 281235 Cow
- Entrez Gene: 478889 Dog
- Entrez Gene: 3417 Human
- Entrez Gene: 100171634 Orangutan
- Entrez Gene: 102166364 Pig
- Omim: 147700 Human
- SwissProt: Q9XSG3 Cow
see all -
Alternative names
- Cytosolic NADP isocitrate dehydrogenase antibody
- Cytosolic NADP-isocitrate dehydrogenase antibody
- Epididymis luminal protein 216 antibody
see all
Images
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Immunoprecipitation - Anti-IDH1 antibody - N-terminal (ab226190)IDH1 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226190 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different rabbit anti-IDH1 antibody at 1 µg/ml.
Lane 1: ab226190 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Datasheets and documents
References
ab226190 has not yet been referenced specifically in any publications.
