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    idh1-antibody-n-terminal-ab226190.pdf

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Signal Transduction Metabolism Energy Metabolism
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Anti-IDH1 antibody - N-terminal (ab226190)

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Immunoprecipitation - Anti-IDH1 antibody - N-terminal (ab226190)

    Key features and details

    • Rabbit polyclonal to IDH1 - N-terminal
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant human IDH1 (mutated R132H) protein (ab198123)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-IDH1 antibody - N-terminal
      See all IDH1 primary antibodies
    • Description

      Rabbit polyclonal to IDH1 - N-terminal
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IPmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Cow, Dog, Pig, Chimpanzee, Orangutan
    • Immunogen

      Synthetic peptide within Human IDH1 aa 1-50 (N terminal). The exact sequence is proprietary. (NP_005887.2).
      Database link: O75874

    • Positive control

      • IP: Jurkat whole cell lysate.
    • General notes

       This product was previously labelled as Isocitrate dehydrogenase

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: Tris citrate/phosphate

      pH 7 to 8
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab226190 was affinity purified using an epitope specific to IDH1 immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Jurkat whole cell lysate (ab30128)
      • Jurkat whole cell lysate (ab7899)
    • Recombinant Protein

      • Recombinant human IDH1 (mutated R132H) protein (ab198123)
    • Related Products

      • Recombinant human IDH1 protein (ab113858)
      • Recombinant human IDH1 protein (ab198096)

    Applications

    Our Abpromise guarantee covers the use of ab226190 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP Use at 2-10 µg/mg of lysate.

    Target

    • Involvement in disease

      Glioma
      Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate.
    • Sequence similarities

      Belongs to the isocitrate and isopropylmalate dehydrogenases family.
    • Post-translational
      modifications

      Acetylation at Lys-374 dramatically reduces catalytic activity.
    • Cellular localization

      Cytoplasm. Peroxisome.
    • Target information above from: UniProt accession O75874 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 460113 Chimpanzee
      • Entrez Gene: 281235 Cow
      • Entrez Gene: 478889 Dog
      • Entrez Gene: 3417 Human
      • Entrez Gene: 100171634 Orangutan
      • Entrez Gene: 102166364 Pig
      • Omim: 147700 Human
      • SwissProt: Q9XSG3 Cow
      • SwissProt: O75874 Human
      • SwissProt: Q5R9C5 Orangutan
      • SwissProt: P20304 Pig
      • Unigene: 593422 Human
      see all
    • Alternative names

      • Cytosolic NADP isocitrate dehydrogenase antibody
      • Cytosolic NADP-isocitrate dehydrogenase antibody
      • Epididymis luminal protein 216 antibody
      • Epididymis secretory protein Li 26 antibody
      • HEL-216 antibody
      • HEL-S-26 antibody
      • ICDH antibody
      • IDCD antibody
      • IDH antibody
      • IDH1 antibody
      • IDHC_HUMAN antibody
      • IDP antibody
      • IDPC antibody
      • Isocitrate dehydrogenase (NADP(+)) 1 cytosolic antibody
      • Isocitrate dehydrogenase [NADP] cytoplasmic antibody
      • Isocitrate dehydrogenase 1 (NADP+) soluble antibody
      • NADP dependent isocitrate dehydrogenase cytosolic antibody
      • NADP dependent isocitrate dehydrogenase peroxisomal antibody
      • NADP(+)-specific ICDH antibody
      • Oxalosuccinate decarboxylase antibody
      • PICD antibody
      see all

    Images

    • Immunoprecipitation - Anti-IDH1 antibody - N-terminal (ab226190)
      Immunoprecipitation - Anti-IDH1 antibody - N-terminal (ab226190)

      IDH1 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226190 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using  a different rabbit anti-IDH1 antibody at 1 µg/ml.

      Lane 1: ab226190 IP in Jurkat whole cell lysate.

      Lane 2: Control IgG IP in Jurkat whole cell lysate.

      Detection:  Chemiluminescence with exposure time of 30 seconds.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab226190? Please let us know so that we can cite the reference in this datasheet.

    ab226190 has not yet been referenced specifically in any publications.

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