Recombinant Anti-IDH1 (mutated R132G) antibody [GMab-r1] (ab264050)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [GMab-r1] to IDH1 (mutated R132G)
- Suitable for: Indirect ELISA, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IDH1 (mutated R132G) antibody [GMab-r1]
See all IDH1 primary antibodies -
Description
Rat monoclonal [GMab-r1] to IDH1 (mutated R132G) -
Host species
Rat -
Tested applications
Suitable for: Indirect ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
GMab-r1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Indirect ELISA |
Use a concentration of 4.058 µg/ml.
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WB |
Use a concentration of 1.266 µg/ml. Predicted molecular weight: 46 kDa.
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Notes |
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Indirect ELISA
Use a concentration of 4.058 µg/ml. |
WB
Use a concentration of 1.266 µg/ml. Predicted molecular weight: 46 kDa. |
Target
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Involvement in disease
Glioma
Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. -
Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
Post-translational
modificationsAcetylation at Lys-374 dramatically reduces catalytic activity. -
Cellular localization
Cytoplasm. Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 3417 Human
- Omim: 147700 Human
- SwissProt: O75874 Human
- Unigene: 593422 Human
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Alternative names
- Cytosolic NADP isocitrate dehydrogenase antibody
- Cytosolic NADP-isocitrate dehydrogenase antibody
- Epididymis luminal protein 216 antibody
see all
Images
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Western blot - Anti-IDH1 (mutated R132 G) antibody [GMab-r1] (ab264050).
ab264050 used at a 1/1000 dilution (1.266µg/ml) followed by Goat Anti-Rat IgG (H+L), HRP) (ab205720) used at a 1/10,000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Lane 1: His-tagged human IDH1 (aa1-414) recombinant protein 10ng
Lane 2: His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein 10ng
Lane 3: His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein 10ng
Lane 4: His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein 10ng
Lane 5: His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein 10ng
Lane 6: His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein 10ng
Lane 7: His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein 10ng
Lane 8: His-tagged human IDH2 (aa40-452) recombinant protein 10ng
Lane 9: His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein 10ng
Lane 10: His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein 10ng
Lane 11: His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein 10ng
Lane 12: His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein 10ng
Lane 13: His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein 10ng
Lane 14: His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein 10ng -
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab264050 has been referenced in 1 publication.
- Kaneko MK et al. Novel monoclonal antibodies GMab-r1 and LMab-1 specifically recognize IDH1-R132G and IDH1-R132L mutations. Monoclon Antib Immunodiagn Immunother 32:224-8 (2013). PubMed: 23750482