Anti-IDH2 antibody (ab109588)
Key features and details
- Goat polyclonal to IDH2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-IDH2 antibody
See all IDH2 primary antibodies -
Description
Goat polyclonal to IDH2 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig
Predicted to work with: Rabbit, Horse, Chicken, Cow, Dog, Macaque monkey -
Immunogen
Synthetic peptide:
CIHGLSNVKLNE
, corresponding to internal sequence amino acids 418-429 of Human IDH2 according to NP_002159.2 -
Positive control
- Human, Mouse or Rat Heart lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 51 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. -
Involvement in disease
D-2-hydroxyglutaric aciduria 2
Glioma
enetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. -
Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
Post-translational
modificationsAcetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 327669 Cow
- Entrez Gene: 3418 Human
- Entrez Gene: 269951 Mouse
- Entrez Gene: 397603 Pig
- Entrez Gene: 361596 Rat
- Omim: 147650 Human
- SwissProt: Q04467 Cow
- SwissProt: P48735 Human
see all -
Alternative names
- D2HGA2 antibody
- ICD-M antibody
- IDH antibody
see all
Images
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All lanes : Anti-IDH2 antibody (ab109588) at 0.1 µg/ml
Lane 1 : Mouse heart tissue lysates
Lane 2 : Rat heart tissue lysates
Lane 3 : Pig heart tissue lysates
Lysates/proteins at 35 µg per lane.
Predicted band size: 51 kDaPrimary incubation was 1 hour.
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Anti-IDH2 antibody (ab109588) at 0.1 µg/ml + Human heart lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Datasheets and documents
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Datasheet download
References (1)
ab109588 has been referenced in 1 publication.
- Feng L et al. Icariside II alleviates oxygen-glucose deprivation and reoxygenation-induced PC12 cell oxidative injury by activating Nrf2/SIRT3 signaling pathway. Biomed Pharmacother 103:9-17 (2018). PubMed: 29635133