Overview

  • Product name
    Anti-IDH2 antibody
    See all IDH2 primary antibodies
  • Description
    Rabbit polyclonal to IDH2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Recombinant fragment within Human IDH2 aa 402-452. The exact sequence is proprietary. NP_002159.2
    Sequence:

    CVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALGR Q


    Database link: P48735

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Whole cell lysate from 293T, HeLa, Jurkat, mouse TCMK-1 and NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab177512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/1000 - 1/5000. Predicted molecular weight: 51 kDa.

Target

  • Function
    Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
  • Involvement in disease
    D-2-hydroxyglutaric aciduria 2
    Glioma
    enetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma.
  • Sequence similarities
    Belongs to the isocitrate and isopropylmalate dehydrogenases family.
  • Post-translational
    modifications
    Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
    • Alternative names
      • D2HGA2 antibody
      • ICD-M antibody
      • IDH antibody
      • IDH2 antibody
      • IDHM antibody
      • IDHP_HUMAN antibody
      • IDP antibody
      • IDPM antibody
      • Isocitrate dehydrogenase [NADP], mitochondrial antibody
      • Isocitrate dehydrogenase 2 (NADP+), mitochondrial antibody
      • mNADP-IDH antibody
      • NADP(+)-specific ICDH antibody
      • Oxalosuccinate decarboxylase antibody
      see all

    Images

    • Western blot - Anti-IDH2 antibody (ab177512)
      Western blot - Anti-IDH2 antibody (ab177512)
      All lanes : Anti-IDH2 antibody (ab177512) at 0.4 µg/ml

      Lane 1 : Whole cell lysate from 293T cells
      Lane 2 : Whole cell lysate from HeLa cells
      Lane 3 : Whole cell lysate from Jurkat cells
      Lane 4 : Whole cell lysate from TCMK-1 cells
      Lane 5 : Whole cell lysate from NIH3T3 cells

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 51 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-IDH2 antibody (ab177512)
      Immunoprecipitation - Anti-IDH2 antibody (ab177512)

      Lane 1: ab177512 at 1µg/ml labeling IDH2 in 293T whole cell lysate immunoprecipitated using ab177512 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

      Lane 2: Control IgG
      Detection: Chemiluminescence with exposure time of 10 seconds.

      .

    References

    ab177512 has not yet been referenced specifically in any publications.

    Publishing research using ab177512? Please let us know so that we can cite the reference in this datasheet.

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