Anti-IDH2 antibody (ab177512)
Key features and details
- Rabbit polyclonal to IDH2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IDH2 antibody
See all IDH2 primary antibodies -
Description
Rabbit polyclonal to IDH2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Recombinant fragment within Human IDH2 aa 402-452. The exact sequence is proprietary. NP_002159.2
Sequence:CVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALGR Q
Database link: P48735 -
Positive control
- Whole cell lysate from 293T, HeLa, Jurkat, mouse TCMK-1 and NIH3T3 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 - 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab177512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 51 kDa. |
Target
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Function
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. -
Involvement in disease
D-2-hydroxyglutaric aciduria 2
Glioma
enetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. -
Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
Post-translational
modificationsAcetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 3418 Human
- Entrez Gene: 397603 Pig
- Omim: 147650 Human
- SwissProt: P48735 Human
- SwissProt: P33198 Pig
- Unigene: 596461 Human
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Alternative names
- D2HGA2 antibody
- ICD-M antibody
- IDH antibody
see all
Images
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Western blot - Anti-IDH2 antibody (ab177512)All lanes : Anti-IDH2 antibody (ab177512) at 0.4 µg/ml
Lane 1 : Whole cell lysate from 293T cells
Lane 2 : Whole cell lysate from HeLa cells
Lane 3 : Whole cell lysate from Jurkat cells
Lane 4 : Whole cell lysate from TCMK-1 cells
Lane 5 : Whole cell lysate from NIH3T3 cells
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-IDH2 antibody (ab177512)Lane 1: ab177512 at 1µg/ml labeling IDH2 in 293T whole cell lysate immunoprecipitated using ab177512 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 10 seconds..
Protocols
Datasheets and documents
References (0)
ab177512 has not yet been referenced specifically in any publications.
