Key features and details
- Rabbit polyclonal to IDH2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-IDH2 antibody
See all IDH2 primary antibodies
DescriptionRabbit polyclonal to IDH2
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
- Whole cell lysate from 293T, HeLa, Jurkat, mouse TCMK-1 and NIH3T3 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 - 8
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab177512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 51 kDa.|
FunctionPlays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
Involvement in diseaseD-2-hydroxyglutaric aciduria 2
enetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma.
Sequence similaritiesBelongs to the isocitrate and isopropylmalate dehydrogenases family.
modificationsAcetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.
- Information by UniProt
- D2HGA2 antibody
- ICD-M antibody
- IDH antibody
All lanes : Anti-IDH2 antibody (ab177512) at 0.4 µg/ml
Lane 1 : Whole cell lysate from 293T cells
Lane 2 : Whole cell lysate from HeLa cells
Lane 3 : Whole cell lysate from Jurkat cells
Lane 4 : Whole cell lysate from TCMK-1 cells
Lane 5 : Whole cell lysate from NIH3T3 cells
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 30 seconds
Lane 1: ab177512 at 1µg/ml labeling IDH2 in 293T whole cell lysate immunoprecipitated using ab177512 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 10 seconds.
ab177512 has not yet been referenced specifically in any publications.