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Signal Transduction Metabolism Energy Metabolism
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-IDH2 antibody [EPR7577] (ab131263)

  • Datasheet
Reviews (1) Submit a question References (12)

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Western blot - Anti-IDH2 antibody [EPR7577] (ab131263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7577] (ab131263)
  • Flow Cytometry - Anti-IDH2 antibody [EPR7577] (ab131263)
  • Western blot - Anti-IDH2 antibody [EPR7577] (ab131263)
  • OI-RD Scanning - Anti-IDH2 antibody [EPR7577] (ab131263)
  • Anti-IDH2 antibody [EPR7577] (ab131263)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7577] to IDH2
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Secondary
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
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Anti-IDH1 antibody [EPR12296] (ab172964)
Protein
Product image
Recombinant human IDH2 protein (ab198092)

View more associated products

Overview

  • Product name

    Anti-IDH2 antibody [EPR7577]
    See all IDH2 primary antibodies
  • Description

    Rabbit monoclonal [EPR7577] to IDH2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues on the C terminal of Human IDH2 (P48735)

  • Positive control

    • WB: MOLT-4, K562, U-87 MG, and HepG2 cell lysates. IHC-P: Human liver tissue. Flow Cyt: MCF7 cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 4.70 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7577
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy

Associated products

  • Alternative Versions

    • PE Anti-IDH2 antibody [EPR7577] (ab212122)
    • Anti-IDH2 antibody [EPR7577] - BSA and Azide free (ab230796)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • MOLT4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant human IDH2 protein (ab198092)

Applications

Our Abpromise guarantee covers the use of ab131263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function

      Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.
    • Involvement in disease

      D-2-hydroxyglutaric aciduria 2
      Glioma
      enetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma.
    • Sequence similarities

      Belongs to the isocitrate and isopropylmalate dehydrogenases family.
    • Post-translational
      modifications

      Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.
    • Cellular localization

      Mitochondrion.
    • Target information above from: UniProt accession P48735 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3418 Human
      • Entrez Gene: 269951 Mouse
      • Entrez Gene: 361596 Rat
      • Omim: 147650 Human
      • SwissProt: P48735 Human
      • SwissProt: P54071 Mouse
      • SwissProt: P56574 Rat
      • Unigene: 596461 Human
      • Unigene: 246432 Mouse
      • Unigene: 290925 Mouse
      • Unigene: 3490 Rat
      see all
    • Alternative names

      • D2HGA2 antibody
      • ICD-M antibody
      • IDH antibody
      • IDH2 antibody
      • IDHM antibody
      • IDHP_HUMAN antibody
      • IDP antibody
      • IDPM antibody
      • Isocitrate dehydrogenase [NADP], mitochondrial antibody
      • Isocitrate dehydrogenase 2 (NADP+), mitochondrial antibody
      • mNADP-IDH antibody
      • NADP(+)-specific ICDH antibody
      • Oxalosuccinate decarboxylase antibody
      see all

    Images

    • Western blot - Anti-IDH2 antibody [EPR7577] (ab131263)
      Western blot - Anti-IDH2 antibody [EPR7577] (ab131263)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: IDH2 knockout HAP1 cell lysate (20 µg)
      Lane 3: K562 cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab131263 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab131263 was shown to recognize IDH2 when IDH2 knockout samples were used, along with additional cross-reactive bands. Wild-type and IDH2 knockout samples were subjected to SDS-PAGE. ab131263 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7577] (ab131263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7577] (ab131263)

      Immunohistochemical analysis of paraffin embedded human liver tissue labelling IDH2 with ab131263 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-IDH2 antibody [EPR7577] (ab131263)
      Flow Cytometry - Anti-IDH2 antibody [EPR7577] (ab131263)

      Overlay histogram showing MCF7 (Human breast adenocarcinoma cell line) cells stained with ab131263 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab131263, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Western blot - Anti-IDH2 antibody [EPR7577] (ab131263)
      Western blot - Anti-IDH2 antibody [EPR7577] (ab131263)
      All lanes : Anti-IDH2 antibody [EPR7577] (ab131263) at 1/1000 dilution

      Lane 1 : MOLT-4 (Human lymphoblastic leukemia cell line) cell lysate
      Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
      Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
      Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 50 kDa

    • OI-RD Scanning - Anti-IDH2 antibody [EPR7577] (ab131263)
      OI-RD Scanning - Anti-IDH2 antibody [EPR7577] (ab131263)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-IDH2 antibody [EPR7577] (ab131263)
      Anti-IDH2 antibody [EPR7577] (ab131263)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (12)

    Publishing research using ab131263? Please let us know so that we can cite the reference in this datasheet.

    ab131263 has been referenced in 12 publications.

    • Seok J  et al. The oncometabolite d-2-hydroxyglutarate induces angiogenic activity through the vascular endothelial growth factor receptor 2 signaling pathway. Int J Oncol 54:753-763 (2019). PubMed: 30483760
    • Wu T  et al. Treadmill Exercise Ameliorates Depression-Like Behavior in the Rats With Prenatal Dexamethasone Exposure: The Role of Hippocampal Mitochondria. Front Neurosci 13:264 (2019). PubMed: 30971882
    • Wang T  et al. SENP1-Sirt3 Signaling Controls Mitochondrial Protein Acetylation and Metabolism. Mol Cell 75:823-834.e5 (2019). PubMed: 31302001
    • McCambridge G  et al. Saturated Fatty Acid Activates T Cell Inflammation Through a Nicotinamide Nucleotide Transhydrogenase (NNT)-Dependent Mechanism. Biomolecules 9:N/A (2019). PubMed: 30823587
    • Dukinfield M  et al. Repurposing an anti-cancer agent for the treatment of hypertrophic heart disease. J Pathol 249:523-535 (2019). PubMed: 31424556
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-IDH2 antibody [EPR7577]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293)
    Permeabilization
    Yes - 0.1% Triton X100
    Specification
    HEK293
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 30 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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