Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-IDH2 antibody [EPR7577] (ab131263)

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Overview

  • Product name

    Anti-IDH2 antibody [EPR7577]
    See all IDH2 primary antibodies

  • Description

    Rabbit monoclonal [EPR7577] to IDH2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide, corresponding to residues on the C terminal of Human IDH2 (P48735)

  • Positive control

    • MOLT4, K562, U87-M, and HepG2 cell lysates; Human liver tissue

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Function

      Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.

    • Involvement in disease

      D-2-hydroxyglutaric aciduria 2
      Glioma
      enetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma.

    • Sequence similarities

      Belongs to the isocitrate and isopropylmalate dehydrogenases family.

    • Post-translational
      modifications

      Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.

    • Cellular localization

      Mitochondrion.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • D2HGA2 antibody
      • ICD-M antibody
      • IDH antibody
      • IDH2 antibody
      • IDHM antibody
      • IDHP_HUMAN antibody
      • IDP antibody
      • IDPM antibody
      • Isocitrate dehydrogenase [NADP], mitochondrial antibody
      • Isocitrate dehydrogenase 2 (NADP+), mitochondrial antibody
      • mNADP-IDH antibody
      • NADP(+)-specific ICDH antibody
      • Oxalosuccinate decarboxylase antibody
      see all

    Images

    • Western blot - Anti-IDH2 antibody [EPR7577] (ab131263)
      Western blot - Anti-IDH2 antibody [EPR7577] (ab131263)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: IDH2 knockout HAP1 cell lysate (20 µg)
      Lane 3: K562 cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab131263 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab131263 was shown to recognize IDH2 when IDH2 knockout samples were used, along with additional cross-reactive bands. Wild-type and IDH2 knockout samples were subjected to SDS-PAGE. ab131263 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7577] (ab131263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH2 antibody [EPR7577] (ab131263)

      Immunohistochemical analysis of paraffin embedded Human liver tissue labelling IDH2 with ab131263 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-IDH2 antibody [EPR7577] (ab131263)
      Flow Cytometry - Anti-IDH2 antibody [EPR7577] (ab131263)
      Overlay histogram showing MCF7 cells stained with ab131263 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab131263, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Western blot - Anti-IDH2 antibody [EPR7577] (ab131263)
      Western blot - Anti-IDH2 antibody [EPR7577] (ab131263)
      All lanes : Anti-IDH2 antibody [EPR7577] (ab131263) at 1/1000 dilution

      Lane 1 : MOLT4 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : U87-M cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 50 kDa

    • Other - Anti-IDH2 antibody [EPR7577] (ab131263)
      Other - Anti-IDH2 antibody [EPR7577] (ab131263)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Seok J  et al. The oncometabolite d-2-hydroxyglutarate induces angiogenic activity through the vascular endothelial growth factor receptor 2 signaling pathway. Int J Oncol 54:753-763 (2019). Read more (PubMed: 30483760) »
    • Wu T  et al. Treadmill Exercise Ameliorates Depression-Like Behavior in the Rats With Prenatal Dexamethasone Exposure: The Role of Hippocampal Mitochondria. Front Neurosci 13:264 (2019). Read more (PubMed: 30971882) »
    See all 9 Publications for this product

    Publishing research using ab131263? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-IDH2 antibody [EPR7577]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293)
    Permeabilization
    Yes - 0.1% Triton X100
    Specification
    HEK293
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

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    Verified customer

    Submitted Sep 30 2017

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