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    idh3a-antibody-ab154886.pdf

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Anti-IDH3A antibody (ab154886)

  • Datasheet
  • SDS
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Western blot - Anti-IDH3A antibody (ab154886)
  • Western blot - Anti-IDH3A antibody (ab154886)
  • Western blot - Anti-IDH3A antibody (ab154886)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3A antibody (ab154886)

Key features and details

  • Rabbit polyclonal to IDH3A
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-IDH3A antibody
    See all IDH3A primary antibodies
  • Description

    Rabbit polyclonal to IDH3A
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 129-366 of Human IDH3A (UniProt ID: P50213).

  • Positive control

    • Raji, mouse brain and rat brain whole cell lysate. Human breast carcinoma tissue.
  • General notes

    Keep as concentrated solution.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • Mouse brain tissue lysate - total protein (ab30151)
    • Rat brain cerebellum tissue lysate - total protein (ab4032)
    • Mouse brain tissue lysate - total protein (0 days) (ab7188)
    • Mouse brain tissue lysate - total protein (14 days) (ab7189)
    • Mouse brain tissue lysate - total protein (7 days) (ab7190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Mouse
Rat
Human
Application Abreviews Notes
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.
  • Cellular localization

    Mitochondrion.
  • Target information above from: UniProt accession P50213 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3419 Human
    • Entrez Gene: 67834 Mouse
    • Entrez Gene: 114096 Rat
    • Omim: 601149 Human
    • SwissProt: P50213 Human
    • SwissProt: Q9D6R2 Mouse
    • SwissProt: Q99NA5 Rat
    • Unigene: 591110 Human
    • Unigene: 279195 Mouse
    • Unigene: 95104 Rat
    see all
  • Alternative names

    • H IDH alpha antibody
    • HIDH alpha antibody
    • IDH3A antibody
    • IDH3A_HUMAN antibody
    • Isocitrate dehydrogenase (NAD+) alpha chain precursor antibody
    • Isocitrate dehydrogenase [NAD] subunit alpha antibody
    • Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial antibody
    • Isocitrate dehydrogenase 3 (NAD+) alpha antibody
    • Isocitrate dehydrogenase antibody
    • Isocitric dehydrogenase antibody
    • Isocitric dehydrogenase subunit alpha antibody
    • mitochondrial antibody
    • NAD antibody
    • NAD(+)-specific ICDH subunit alpha antibody
    • NAD(H) specific isocitrate dehydrogenase alpha subunit precursor antibody
    • NAD+ specific ICDH antibody
    • NADsubunit alpha mitochondrial precursor antibody
    • OTTHUMP00000184906 antibody
    see all

Images

  • Western blot - Anti-IDH3A antibody (ab154886)
    Western blot - Anti-IDH3A antibody (ab154886)
    Anti-IDH3A antibody (ab154886) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Western blot - Anti-IDH3A antibody (ab154886)
    Western blot - Anti-IDH3A antibody (ab154886)
    Anti-IDH3A antibody (ab154886) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Western blot - Anti-IDH3A antibody (ab154886)
    Western blot - Anti-IDH3A antibody (ab154886)
    Anti-IDH3A antibody (ab154886) at 1/10000 dilution + Rat brain whole cell lysate/extract at 50 µg

    Predicted band size: 40 kDa



    10% SDS-PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3A antibody (ab154886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3A antibody (ab154886)
    Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue, labeling IDH3A using ab154886 at 1/250 dilution.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab154886? Please let us know so that we can cite the reference in this datasheet.

    ab154886 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-IDH3A antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Horse Tissue lysate - whole (Liver)
    Gel Running Conditions
    Non-reduced Denaturing (12 %)
    Loading amount
    60 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 23 2017

    Western blot abreview for Anti-IDH3A antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (12.5% Acrylamide)
    Sample
    Pig Tissue lysate - other (Muscle Sarcoplasm)
    Specification
    Muscle Sarcoplasm
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Dr. Shannon Cruzen

    Verified customer

    Submitted Oct 06 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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