Anti-IDH3A antibody (ab154886)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-IDH3A antibody
See all IDH3A primary antibodies -
Description
Rabbit polyclonal to IDH3A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 129-366 of Human IDH3A (UniProt ID: P50213).
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Positive control
- Raji, mouse brain and rat brain whole cell lysate. Human breast carcinoma tissue.
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General notes
Keep as concentrated solution.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- Raji whole cell lysate (ab30124)
- Mouse brain tissue lysate - total protein (ab30151)
- Rat brain cerebellum tissue lysate - total protein (ab4032)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
Applications
Our Abpromise guarantee covers the use of ab154886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 40 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 3419 Human
- Entrez Gene: 67834 Mouse
- Entrez Gene: 114096 Rat
- Omim: 601149 Human
- SwissProt: P50213 Human
- SwissProt: Q9D6R2 Mouse
- SwissProt: Q99NA5 Rat
- Unigene: 591110 Human
see all -
Alternative names
- H IDH alpha antibody
- HIDH alpha antibody
- IDH3A antibody
see all
Images
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Western blot - Anti-IDH3A antibody (ab154886)Anti-IDH3A antibody (ab154886) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 40 kDa
10% SDS PAGE -
Western blot - Anti-IDH3A antibody (ab154886)Anti-IDH3A antibody (ab154886) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 40 kDa
10% SDS PAGE -
Western blot - Anti-IDH3A antibody (ab154886)Anti-IDH3A antibody (ab154886) at 1/10000 dilution + Rat brain whole cell lysate/extract at 50 µg
Predicted band size: 40 kDa
10% SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3A antibody (ab154886)Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue, labeling IDH3A using ab154886 at 1/250 dilution.
References
ab154886 has not yet been referenced specifically in any publications.
