Overview

  • Product name
    Anti-IDH3A antibody
    See all IDH3A primary antibodies
  • Description
    Rabbit polyclonal to IDH3A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 129-366 of Human IDH3A (UniProt ID: P50213).

  • Positive control
    • Raji, mouse brain and rat brain whole cell lysate. Human breast carcinoma tissue.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities
    Belongs to the isocitrate and isopropylmalate dehydrogenases family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • H IDH alpha antibody
    • HIDH alpha antibody
    • IDH3A antibody
    • IDH3A_HUMAN antibody
    • Isocitrate dehydrogenase (NAD+) alpha chain precursor antibody
    • Isocitrate dehydrogenase [NAD] subunit alpha antibody
    • Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial antibody
    • Isocitrate dehydrogenase 3 (NAD+) alpha antibody
    • Isocitrate dehydrogenase antibody
    • Isocitric dehydrogenase antibody
    • Isocitric dehydrogenase subunit alpha antibody
    • mitochondrial antibody
    • NAD antibody
    • NAD(+)-specific ICDH subunit alpha antibody
    • NAD(H) specific isocitrate dehydrogenase alpha subunit precursor antibody
    • NAD+ specific ICDH antibody
    • NADsubunit alpha mitochondrial precursor antibody
    • OTTHUMP00000184906 antibody
    see all

Images

  • Western blot - Anti-IDH3A antibody (ab154886)
    Western blot - Anti-IDH3A antibody (ab154886)
    Anti-IDH3A antibody (ab154886) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Western blot - Anti-IDH3A antibody (ab154886)
    Western blot - Anti-IDH3A antibody (ab154886)
    Anti-IDH3A antibody (ab154886) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Western blot - Anti-IDH3A antibody (ab154886)
    Western blot - Anti-IDH3A antibody (ab154886)
    Anti-IDH3A antibody (ab154886) at 1/10000 dilution + Rat brain whole cell lysate/extract at 50 µg

    Predicted band size: 40 kDa



    10% SDS-PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3A antibody (ab154886)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3A antibody (ab154886)
    Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue, labeling IDH3A using ab154886 at 1/250 dilution.

References

ab154886 has not yet been referenced specifically in any publications.

Publishing research using ab154886? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-IDH3A antibody

 Excellent
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Horse Tissue lysate - whole (Liver)
Gel Running Conditions
Non-reduced Denaturing (12 %)
Loading amount
60 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted May 23 2017

Western blot abreview for Anti-IDH3A antibody

 Average
Abreviews
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12.5% Acrylamide)
Sample
Pig Tissue lysate - other (Muscle Sarcoplasm)
Specification
Muscle Sarcoplasm
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
abreview image
Username

Dr. Shannon Cruzen

Verified customer

Submitted Oct 06 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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