Overview

  • Product name
  • Description
    Rabbit polyclonal to IDH3A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 129-366 of Human IDH3A (UniProt ID: P50213).

  • Positive control
    • Raji, mouse brain and rat brain whole cell lysate. Human breast carcinoma tissue.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities
    Belongs to the isocitrate and isopropylmalate dehydrogenases family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • H IDH alpha antibody
    • HIDH alpha antibody
    • IDH3A antibody
    • IDH3A_HUMAN antibody
    • Isocitrate dehydrogenase (NAD+) alpha chain precursor antibody
    • Isocitrate dehydrogenase [NAD] subunit alpha antibody
    • Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial antibody
    • Isocitrate dehydrogenase 3 (NAD+) alpha antibody
    • Isocitrate dehydrogenase antibody
    • Isocitric dehydrogenase antibody
    • Isocitric dehydrogenase subunit alpha antibody
    • mitochondrial antibody
    • NAD antibody
    • NAD(+)-specific ICDH subunit alpha antibody
    • NAD(H) specific isocitrate dehydrogenase alpha subunit precursor antibody
    • NAD+ specific ICDH antibody
    • NADsubunit alpha mitochondrial precursor antibody
    • OTTHUMP00000184906 antibody
    see all

Images

  • Anti-IDH3A antibody (ab154886) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Anti-IDH3A antibody (ab154886) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Anti-IDH3A antibody (ab154886) at 1/10000 dilution + Rat brain whole cell lysate/extract at 50 µg

    Predicted band size: 40 kDa



    10% SDS-PAGE
  • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue, labeling IDH3A using ab154886 at 1/250 dilution.

References

ab154886 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Horse Tissue lysate - whole (Liver)
Gel Running Conditions
Non-reduced Denaturing (12 %)
Loading amount
60 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 23 2017

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12.5% Acrylamide)
Sample
Pig Tissue lysate - other (Muscle Sarcoplasm)
Specification
Muscle Sarcoplasm
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Shannon Cruzen

Verified customer

Submitted Oct 06 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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