Anti-IDH3A antibody (ab154886)
Key features and details
- Rabbit polyclonal to IDH3A
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IDH3A antibody
See all IDH3A primary antibodies -
Description
Rabbit polyclonal to IDH3A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseRatHuman
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Immunogen
Recombinant fragment corresponding to a region within amino acids 129-366 of Human IDH3A (UniProt ID: P50213).
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Positive control
- Raji, mouse brain and rat brain whole cell lysate. Human breast carcinoma tissue.
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General notes
Keep as concentrated solution.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- Raji whole cell lysate (ab30124)
- Mouse brain tissue lysate - total protein (ab30151)
- Rat brain cerebellum tissue lysate - total protein (ab4032)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Mouse
Rat
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 3419 Human
- Entrez Gene: 67834 Mouse
- Entrez Gene: 114096 Rat
- Omim: 601149 Human
- SwissProt: P50213 Human
- SwissProt: Q9D6R2 Mouse
- SwissProt: Q99NA5 Rat
- Unigene: 591110 Human
see all -
Alternative names
- H IDH alpha antibody
- HIDH alpha antibody
- IDH3A antibody
see all
Images
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Western blot - Anti-IDH3A antibody (ab154886)Anti-IDH3A antibody (ab154886) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 40 kDa
10% SDS PAGE -
Western blot - Anti-IDH3A antibody (ab154886)Anti-IDH3A antibody (ab154886) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 40 kDa
10% SDS PAGE -
Western blot - Anti-IDH3A antibody (ab154886)Anti-IDH3A antibody (ab154886) at 1/10000 dilution + Rat brain whole cell lysate/extract at 50 µg
Predicted band size: 40 kDa
10% SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3A antibody (ab154886)Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue, labeling IDH3A using ab154886 at 1/250 dilution.
Protocols
References (0)
ab154886 has not yet been referenced specifically in any publications.
