Overview

  • Product name

  • Description

    Rabbit polyclonal to IDH3A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Xenopus laevis, Zebrafish, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human IDH3A (internal sequence). The exact sequence is proprietary.
    Database link: P50213

  • Positive control

    • WB: Mouse and rat brain tissue lysate: HepG2 whole cell lysate (ab7900). IHC-P: Human lung SCC tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-IDH3A antibody (ab228596) at 1/10000 dilution + Rat brain tissue lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 40 kDa



    10% SDS-PAGE

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for IDH3A (green) using ab228596 at 1/500 dilution in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes.

    Blue: Hoechst 33342 staining.

  • Paraffin-embedded human lung SCC tissue stained for IDH3A with ab228596 at 1/100 dilution in immunohistochemical analysis

  • Anti-IDH3A antibody (ab228596) at 1/10000 dilution + Mouse brain tissue lysate at 20 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 40 kDa



    10% SDS-PAGE

  • Anti-IDH3A antibody (ab228596) at 1/500 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 40 kDa



    12% SDS-PAGE

References

ab228596 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228596.
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