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  1. Link

    idh3b-antibody-ab247089.pdf

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Signal Transduction Metabolism Energy Metabolism
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Anti-IDH3B antibody (ab247089)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-IDH3B antibody (ab247089)
  • Western blot - Anti-IDH3B antibody (ab247089)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)

Key features and details

  • Rabbit polyclonal to IDH3B
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-IDH3B antibody
    See all IDH3B primary antibodies
  • Description

    Rabbit polyclonal to IDH3B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Cow, Cynomolgus monkey, Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human IDH3B aa 67-157.
    Sequence:

    VKEVFKAAAVPVEFQEHHLSEVQNMASEEKLEQVLSSMKENKVAIIGKIH TPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNN


    Database link: O43837
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human parathyroid gland, pancreas, kidney, placenta and testis tissue. WB: RT4 and U-251 MG cell lysates; Human tonsil lysate. ICC/IF: A549 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

Applications

Our Abpromise guarantee covers the use of ab247089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

WB Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 42 kDa.

Target

  • Involvement in disease

    Defects in IDH3B are the cause of retinitis pigmentosa type 46 (RP46) [MIM:612572]. RP is a retinal dystrophy belonging to the group of pigmentary retinopathies. RP is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
  • Sequence similarities

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.
  • Cellular localization

    Mitochondrion.
  • Target information above from: UniProt accession O43837 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 613338 Cow
    • Entrez Gene: 102129545 Cynomolgus monkey
    • Entrez Gene: 3420 Human
    • Entrez Gene: 100172344 Orangutan
    • Entrez Gene: 94173 Rat
    • Omim: 604526 Human
    • SwissProt: O77784 Cow
    • SwissProt: Q28479 Cynomolgus monkey
    • SwissProt: O43837 Human
    • SwissProt: Q5RBT4 Orangutan
    • SwissProt: Q68FX0 Rat
    • Unigene: 436405 Human
    • Unigene: 1093 Rat
    see all
  • Alternative names

    • FLJ11043 antibody
    • H-IDHB antibody
    • Idh3B antibody
    • IDH3B_HUMAN antibody
    • Isocitrate dehydrogenase [NAD] subunit beta antibody
    • Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial antibody
    • isocitrate dehydrogenase 3, beta subunit antibody
    • Isocitric dehydrogenase antibody
    • Isocitric dehydrogenase subunit beta antibody
    • MGC903 antibody
    • mitochondrial antibody
    • NAD(+)-specific ICDH antibody
    • NAD(+)-specific ICDH subunit beta antibody
    • NAD+-specific isocitrate dehydrogenase b subunit antibody
    • NAD+-specific isocitrate dehydrogenase beta antibody
    • OTTHUMP00000030023 antibody
    • OTTHUMP00000030024 antibody
    • RP46 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-IDH3B antibody (ab247089)
    Immunocytochemistry/ Immunofluorescence - Anti-IDH3B antibody (ab247089)

    PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells stained for IDH3B (green) using ab247089 at 4 µg/ml in ICC/IF.

  • Western blot - Anti-IDH3B antibody (ab247089)
    Western blot - Anti-IDH3B antibody (ab247089)
    All lanes : Anti-IDH3B antibody (ab247089) at 0.4 µg/ml

    Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
    Lane 2 : U-251 MG (human rain glioma cell line) cell lysate
    Lane 3 : Human plasma (IgG/HSA depleted)
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Predicted band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)

    Formalin-fixed, paraffin-embedded human testis tissue stained for IDH3B with ab247089 at a 1/2500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)

    Formalin-fixed, paraffin-embedded human kidney tissue stained for IDH3B with ab247089 at a 1/2500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)

    Formalin-fixed, paraffin-embedded human pancreas tissue stained for IDH3B with ab247089 at a 1/2500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for IDH3B with ab247089 at a 1/2500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)

    Formalin-fixed, paraffin-embedded human parathyroid gland tissue stained for IDH3B with ab247089 at a 1/2500 dilution in immunohistochemical analysis.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab247089? Please let us know so that we can cite the reference in this datasheet.

    ab247089 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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