Product nameAnti-IDH3B antibody
See all IDH3B primary antibodies
DescriptionRabbit polyclonal to IDH3B
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Cow, Cynomolgus monkey, Orangutan
- IHC-P: Human parathyroid gland tissue. WB: RT4 and U-251 MG cell lysates; Human tonsil lysate. ICC/IF: A549 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab247089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.4 µg/ml. Predicted molecular weight: 42 kDa.|
Involvement in diseaseDefects in IDH3B are the cause of retinitis pigmentosa type 46 (RP46) [MIM:612572]. RP is a retinal dystrophy belonging to the group of pigmentary retinopathies. RP is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Sequence similaritiesBelongs to the isocitrate and isopropylmalate dehydrogenases family.
- Information by UniProt
- FLJ11043 antibody
- H-IDHB antibody
- Idh3B antibody
Paraffin-embedded human parathyroid gland tissue stained for IDH3B using ab247089 at 1/2500 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells stained for IDH3B (green) using ab247089 at 4 µg/ml in ICC/IF.
All lanes : Anti-IDH3B antibody (ab247089) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human rain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 42 kDa
Paraffin-embedded human pancreas tissue stained for IDH3B using ab247089 at 1/2500 dilution in immunohistochemical analysis.
ab247089 has not yet been referenced specifically in any publications.