Key features and details
- Goat polyclonal to IDH3G
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Product nameAnti-IDH3G antibody
See all IDH3G primary antibodies
DescriptionGoat polyclonal to IDH3G
Specificityab118289 is expected to recognize both reported isoforms of IDH3G (NP_004126.1; NP_777358.1).
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human, Pig
Predicted to work with: Cow, Dog
- MCF7 lysate; Human, Mouse and Rat heart lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab118289 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab118289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 43 kDa).
1 hour primary incubation is recommended for this product.
Sequence similaritiesBelongs to the isocitrate and isopropylmalate dehydrogenases family.
- Information by UniProt
- H IDHG antibody
- IDH gamma antibody
- IDH3G antibody
All lanes : Anti-IDH3G antibody (ab118289) at 1 µg/ml
Lane 1 : Rat heart lysate
Lane 2 : Pig heart lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 43 kDa
Anti-IDH3G antibody (ab118289) at 0.5 µg/ml + MCF7 lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
ab118289 has not yet been referenced specifically in any publications.