Anti-IDOL antibody (ab74562)
Key features and details
- Rabbit polyclonal to IDOL
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-IDOL antibody -
Description
Rabbit polyclonal to IDOL -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human IDOL (internal sequence). Immunogen Range:161-210
Database link: Q8WY64 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
WB | (3) |
1/500 - 1/1000. Predicted molecular weight: 50 kDa.
|
IHC-P | (1) |
1/50 - 1/100.
|
Notes |
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ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 50 kDa. |
IHC-P
1/50 - 1/100. |
Target
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Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. -
Tissue specificity
Ubiquitously expressed. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 FERM domain.
Contains 1 RING-type zinc finger. -
Developmental stage
Expressed in fetal tissues and higher levels were detected in placenta and fetal lung. -
Domain
The RING domain mediates ubiquitination and the neurite outgrowth inhibitory activity. -
Post-translational
modificationsAutoubiquitinated. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 29116 Human
- Entrez Gene: 218203 Mouse
- Omim: 610082 Human
- SwissProt: Q8WY64 Human
- SwissProt: Q8BM54 Mouse
- Unigene: 484738 Human
- Unigene: 212855 Mouse
- Unigene: 481228 Mouse
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Alternative names
- BM-023 antibody
- BZF1 antibody
- E3 ubiquitin protein ligase MYLIP antibody
see all
Images
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All lanes : Anti-IDOL antibody (ab74562) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from Jurkat cells
Lane 3 : extracts from Jurkat cells with immunising peptide at 10 µg
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry analysis of IDOL in paraffin embedded human brain tissue using 1/50 ab74562.
Left image: untreated sample.
Right image: sample treated with immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab74562 has been referenced in 10 publications.
- Zhao L et al. Long non-coding RNA TUSC8 inhibits breast cancer growth and metastasis via miR-190b-5p/MYLIP axis. Aging (Albany NY) 12:2974-2991 (2020). PubMed: 32039833
- Ito S et al. CNPY2 inhibits MYLIP-mediated AR protein degradation in prostate cancer cells. Oncotarget 9:17645-17655 (2018). PubMed: 29707137
- Leitch EK et al. Inhibition of low-density lipoprotein receptor degradation with a cyclic peptide that disrupts the homodimerization of IDOL E3 ubiquitin ligase. Chem Sci 9:5957-5966 (2018). PubMed: 30079210
- Zhao L et al. miR-19b promotes breast cancer metastasis through targeting MYLIP and its related cell adhesion molecules. Oncotarget 8:64330-64343 (2017). PubMed: 28969074
- Zhou Y et al. Docosahexanoic acid modifies low-density lipoprotein receptor abundance in HepG2 cells via suppression of the LXRa-Idol pathway. Mol Med Rep 11:2329-33 (2015). PubMed: 25394495
- Scotti E et al. IDOL stimulates clathrin-independent endocytosis and multivesicular body-mediated lysosomal degradation of the low-density lipoprotein receptor. Mol Cell Biol 33:1503-14 (2013). WB ; Human . PubMed: 23382078
- Do HT et al. Reciprocal regulation of very low density lipoprotein receptors (VLDLRs) in neurons by brain-derived neurotrophic factor (BDNF) and Reelin: involvement of the E3 ligase Mylip/Idol. J Biol Chem 288:29613-20 (2013). WB ; Rat . PubMed: 23990472
- Do HT et al. Fibroblast Growth Factor-21 (FGF21) Regulates Low-density Lipoprotein Receptor (LDLR) Levels in Cells via the E3-ubiquitin Ligase Mylip/Idol and the Canopy2 (Cnpy2)/Mylip-interacting Saposin-like Protein (Msap). J Biol Chem 287:12602-11 (2012). WB ; Mouse . PubMed: 22378787
- Hu X et al. LXRß activation increases intestinal cholesterol absorption, leading to an atherogenic lipoprotein profile. J Intern Med 272:452-464 (2012). WB ; Mouse . PubMed: 22329358
- Ishibashi M et al. Reduced VLDL clearance in Apoe(-/-)Npc1(-/-) mice is associated with increased Pcsk9 and Idol expression and decreased hepatic LDL-receptor levels. J Lipid Res 51:2655-63 (2010). WB ; Mouse . PubMed: 20562239