Product nameAnti-IDOL antibody
See all IDOL primary antibodies
DescriptionRabbit polyclonal to IDOL
Specificityab74562 detects endogenous levels of total IDOL protein.
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human IDOL (internal sequence). Immunogen Range:161-210
Database link: Q8WY64
- IHC-P: human brain tissue WB: extracts from HeLa cells and Jurkat cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Our Abpromise guarantee covers the use of ab74562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 50 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionE3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR.
Tissue specificityUbiquitously expressed.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 FERM domain.
Contains 1 RING-type zinc finger.
Developmental stageExpressed in fetal tissues and higher levels were detected in placenta and fetal lung.
DomainThe RING domain mediates ubiquitination and the neurite outgrowth inhibitory activity.
- Information by UniProt
- BM-023 antibody
- BZF1 antibody
- E3 ubiquitin protein ligase MYLIP antibody
All lanes : Anti-IDOL antibody (ab74562) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from Jurkat cells
Lane 3 : extracts from Jurkat cells with immunising peptide at 10 µg
Lysates/proteins at 10 µg per lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry analysis of IDOL in paraffin embedded human brain tissue using 1/50 ab74562.
Left image: untreated sample.
Right image: sample treated with immunising peptide.
This product has been referenced in:
- Ito S et al. CNPY2 inhibits MYLIP-mediated AR protein degradation in prostate cancer cells. Oncotarget 9:17645-17655 (2018). Read more (PubMed: 29707137) »
- Leitch EK et al. Inhibition of low-density lipoprotein receptor degradation with a cyclic peptide that disrupts the homodimerization of IDOL E3 ubiquitin ligase. Chem Sci 9:5957-5966 (2018). Read more (PubMed: 30079210) »